Cannabidiol alleviates LPS‐inhibited odonto/osteogenic differentiation in human dental pulp stem cells in vitro

牙髓干细胞 大麻酚 细胞生物学 细胞生长 细胞凋亡 干细胞 下调和上调 化学 小干扰RNA 成牙骨质细胞 生物 牙骨质 细胞培养 牙科 医学 转染 生物化学 大麻 精神科 遗传学 牙本质 基因
作者
Chatvadee Kornsuthisopon,Ajjima Chansaenroj,Ravipha Suwittayarak,Suphalak Phothichailert,Khunakon Usarprom,Apicha Srikacha,Sornkanok Vimolmangkang,Chaloemrit Phrueksotsai,Lakshman P. Samaranayake,Thanaphum Osathanon
出处
期刊:International Endodontic Journal [Wiley]
被引量:1
标识
DOI:10.1111/iej.14183
摘要

Abstract Aim Cannabidiol (CBD), derived from the Cannabis sativa plant, exhibits benefits in potentially alleviating a number of oral and dental pathoses, including pulpitis and periodontal diseases. This study aimed to explore the impact of CBD on several traits of human dental pulp stem cells (hDPSC), such as their proliferation, apoptosis, migration and odonto/osteogenic differentiation. Methodology hDPSCs were harvested from human dental pulp tissues. The cells were treated with CBD at concentrations of 1.25, 2.5, 5, 10, 25 and 50 μg/mL. Cell responses in terms of cell proliferation, colony‐forming unit, cell cycle progression, cell migration, apoptosis and odonto/osteogenic differentiation of hDPSCs were assessed in the normal culture condition and P. gingivalis lipopolysaccharide (LPS)‐induced ‘inflammatory’ milieus. RNA sequencing and proteomic analysis were performed to predict target pathways impacted by CBD. Results CBD minimally affects hDPSCs' behaviour under normal culture growth milieu in normal conditions. However, an optimal concentration of 1.25 μg/mL CBD significantly countered the harmful effects of LPS, indicated by the promoting cell proliferation and restoring the odonto/osteogenic differentiation potential of hDPSCs under LPS‐treated conditions. The proteomic analysis demonstrated that several proteins involved in cell proliferation and differentiation were upregulated following CBD exposure, including CCL8, CDC42 and KFL5. RNA sequencing data indicated that CBD upregulated the Notch signalling pathway. In an inhibitory experiment, DAPT, a Notch inhibitor, reduced the effect of CBD‐rescued LPS‐attenuated mineralization in hDPSCs, suggesting that CBD potentially mediates Notch activation to exert its impact on odonto/osteogenic differentiation of hDPSCs. Conclusions CBD recovers the proliferation and survival of hDPSCs following exposure to LPS. Additionally, we report that CBD‐mediated Notch activation effectively restores the odonto/osteogenic differentiation ability of hDPSCs under inflamed conditions. These results underscore the potential role of CBD as a therapeutic option to enhance dentine regeneration.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
翁雁丝完成签到 ,获得积分10
2秒前
郭义敏完成签到,获得积分0
2秒前
gyf完成签到,获得积分10
5秒前
李保龙完成签到 ,获得积分10
6秒前
10秒前
LJJ完成签到 ,获得积分10
11秒前
量子星尘发布了新的文献求助10
14秒前
阿姨洗铁路完成签到 ,获得积分10
19秒前
抹不掉的记忆完成签到,获得积分10
21秒前
21秒前
余杭村王小虎完成签到,获得积分10
22秒前
韭黄完成签到,获得积分20
26秒前
jeffrey完成签到,获得积分10
26秒前
Rondab应助机灵枕头采纳,获得10
32秒前
佳无夜完成签到,获得积分10
37秒前
摆哥完成签到,获得积分10
41秒前
66完成签到,获得积分10
46秒前
zlqq完成签到 ,获得积分10
46秒前
Hardskills发布了新的文献求助10
49秒前
50秒前
之_ZH完成签到 ,获得积分10
58秒前
gds2021完成签到 ,获得积分10
1分钟前
你好呀嘻嘻完成签到 ,获得积分10
1分钟前
梅特卡夫完成签到,获得积分10
1分钟前
熊雅完成签到,获得积分10
1分钟前
1分钟前
睡到自然醒完成签到 ,获得积分10
1分钟前
cis2014完成签到,获得积分10
1分钟前
独特的大有完成签到 ,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
1分钟前
xingyi完成签到,获得积分10
1分钟前
1分钟前
舒心的秋荷完成签到 ,获得积分10
1分钟前
zz123发布了新的文献求助10
1分钟前
liaomr完成签到 ,获得积分10
1分钟前
粗犷的灵松完成签到,获得积分10
1分钟前
吃小孩的妖怪完成签到 ,获得积分10
1分钟前
ncuwzq完成签到,获得积分10
1分钟前
yshj完成签到 ,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038112
求助须知:如何正确求助?哪些是违规求助? 3575788
关于积分的说明 11373801
捐赠科研通 3305604
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022