Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Ivy完成签到,获得积分10
刚刚
tz完成签到,获得积分10
刚刚
杏梨完成签到 ,获得积分10
1秒前
朝北完成签到 ,获得积分10
1秒前
yy发布了新的文献求助10
1秒前
Dqa发布了新的文献求助10
2秒前
ocean完成签到,获得积分10
2秒前
wcw完成签到 ,获得积分10
3秒前
读研好难发布了新的文献求助10
4秒前
having完成签到,获得积分10
4秒前
Www1231发布了新的文献求助10
4秒前
仲乔妹完成签到,获得积分10
5秒前
5秒前
田田田田完成签到,获得积分10
5秒前
脑洞疼应助司空珩采纳,获得10
5秒前
我是大霖子完成签到,获得积分10
6秒前
JiegeSCI完成签到,获得积分10
6秒前
limecho完成签到,获得积分10
6秒前
ny960发布了新的文献求助10
6秒前
Hello应助NING采纳,获得10
7秒前
汤汤完成签到,获得积分10
8秒前
wqy完成签到 ,获得积分10
8秒前
范白容完成签到 ,获得积分10
8秒前
sia完成签到 ,获得积分0
9秒前
悦耳紫霜发布了新的文献求助10
10秒前
黑猫乾杯完成签到,获得积分0
10秒前
Rosaline完成签到 ,获得积分10
11秒前
一昂杨完成签到,获得积分10
11秒前
零123完成签到,获得积分20
11秒前
12秒前
周周完成签到,获得积分10
12秒前
靓丽念薇完成签到,获得积分10
13秒前
庄彧完成签到 ,获得积分10
13秒前
summer完成签到,获得积分10
13秒前
充电宝应助玄同采纳,获得10
14秒前
三七完成签到 ,获得积分10
15秒前
丰知然应助开心的白昼采纳,获得10
15秒前
充电宝应助零123采纳,获得10
17秒前
lizhaonian完成签到,获得积分10
17秒前
JS完成签到,获得积分10
17秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196879
求助须知:如何正确求助?哪些是违规求助? 2845528
关于积分的说明 8054887
捐赠科研通 2510263
什么是DOI,文献DOI怎么找? 1342345
科研通“疑难数据库(出版商)”最低求助积分说明 639355
邀请新用户注册赠送积分活动 608690