Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
pluto应助飞跃海龙采纳,获得10
1秒前
Cheryy完成签到,获得积分10
2秒前
陆肥肥完成签到 ,获得积分10
2秒前
CodeCraft应助211采纳,获得10
2秒前
大模型应助东花坊时雨采纳,获得10
2秒前
Akim应助悬壶济世之骨科采纳,获得10
3秒前
4秒前
李健应助goku采纳,获得10
4秒前
爆米花应助喜欢划水的仔采纳,获得10
5秒前
小马甲应助英俊若灵采纳,获得10
5秒前
5秒前
清茶韵心发布了新的文献求助10
7秒前
8秒前
8秒前
深情安青应助ECCE采纳,获得10
8秒前
小二郎应助嘟嘟金子采纳,获得10
9秒前
RoyChen发布了新的文献求助10
9秒前
斯文败类应助醉熏的蛋挞采纳,获得30
9秒前
10秒前
JamesPei应助aaaaa采纳,获得10
10秒前
10秒前
10秒前
英俊的铭应助小赵采纳,获得10
12秒前
12秒前
YYY发布了新的文献求助10
13秒前
13秒前
kkkkkkk_应助簪花似魁收藏采纳,获得10
13秒前
Summertrain发布了新的文献求助30
13秒前
zzlark发布了新的文献求助10
14秒前
14秒前
15秒前
15秒前
田様应助AMQAQMA采纳,获得30
15秒前
ZHANG发布了新的文献求助10
15秒前
追忆发布了新的文献求助10
16秒前
17秒前
斯文可仁发布了新的文献求助10
17秒前
benben应助cfder采纳,获得10
18秒前
18秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mixed-anion Compounds 600
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
China as a Double-Bind Regulatory State: How Internet Regulators’ Predicament Produces Regulatees’ Autonomy 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3199060
求助须知:如何正确求助?哪些是违规求助? 2847958
关于积分的说明 8065446
捐赠科研通 2512681
什么是DOI,文献DOI怎么找? 1344652
科研通“疑难数据库(出版商)”最低求助积分说明 639845
邀请新用户注册赠送积分活动 609663