亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
15秒前
现代青枫应助owlhealth采纳,获得10
27秒前
现代青枫应助白华苍松采纳,获得10
40秒前
42秒前
1分钟前
不安萃发布了新的文献求助10
1分钟前
栗悟饭完成签到,获得积分10
1分钟前
不安萃完成签到,获得积分20
1分钟前
现代青枫应助白华苍松采纳,获得10
1分钟前
Yifan2024完成签到,获得积分10
2分钟前
2分钟前
白华苍松发布了新的文献求助10
3分钟前
小马甲应助白华苍松采纳,获得10
3分钟前
3分钟前
程静亭发布了新的文献求助10
3分钟前
程静亭完成签到,获得积分20
4分钟前
4分钟前
owlhealth完成签到,获得积分10
5分钟前
owlhealth发布了新的文献求助10
5分钟前
5分钟前
李健应助下一个采纳,获得10
6分钟前
6分钟前
高兴小懒虫关注了科研通微信公众号
6分钟前
下一个关注了科研通微信公众号
6分钟前
7分钟前
7分钟前
7分钟前
下一个发布了新的文献求助10
7分钟前
7分钟前
杀了几只三七完成签到,获得积分10
7分钟前
7分钟前
8分钟前
hwen1998完成签到 ,获得积分10
8分钟前
8分钟前
科研通AI2S应助一个如茶采纳,获得10
8分钟前
海洋球发布了新的文献求助10
8分钟前
海洋球完成签到,获得积分10
8分钟前
9分钟前
9分钟前
Tumumu完成签到,获得积分10
10分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
保险藏宝图 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3186712
求助须知:如何正确求助?哪些是违规求助? 2836935
关于积分的说明 8011948
捐赠科研通 2499373
什么是DOI,文献DOI怎么找? 1334305
科研通“疑难数据库(出版商)”最低求助积分说明 637154
邀请新用户注册赠送积分活动 605047