Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
lvsehx发布了新的文献求助10
2秒前
hangongyishan完成签到,获得积分10
3秒前
科研通AI2S应助烂漫的幻梅采纳,获得10
4秒前
上官若男应助马骁采纳,获得10
5秒前
小周同学发布了新的文献求助10
6秒前
6秒前
7秒前
8秒前
Jasper应助动听的薯条采纳,获得10
9秒前
单薄广山发布了新的文献求助10
11秒前
11秒前
guangming完成签到,获得积分20
12秒前
12秒前
甜甜十三发布了新的文献求助10
13秒前
老实的半山完成签到,获得积分10
14秒前
找不到完成签到,获得积分0
14秒前
大模型应助司空晓山采纳,获得10
14秒前
Yimim发布了新的文献求助10
16秒前
研友_ZAVbe8发布了新的文献求助60
17秒前
17秒前
ddd发布了新的文献求助10
17秒前
卡沙巴完成签到,获得积分10
18秒前
无花果应助提拉米苏采纳,获得10
19秒前
xtt完成签到,获得积分10
20秒前
21秒前
马骁发布了新的文献求助10
22秒前
鱼鱼鱼给鱼鱼鱼的求助进行了留言
24秒前
iperper发布了新的文献求助10
24秒前
26秒前
Yimim完成签到,获得积分10
26秒前
小周同学发布了新的文献求助10
27秒前
27秒前
虚幻的雪巧完成签到,获得积分10
28秒前
哇咔咔应助沐风采纳,获得10
28秒前
iperper完成签到,获得积分10
29秒前
谈笑间发布了新的文献求助10
31秒前
NexusExplorer应助Dylan采纳,获得10
32秒前
提拉米苏发布了新的文献求助10
32秒前
赘婿应助淡然的书本采纳,获得10
33秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3188290
求助须知:如何正确求助?哪些是违规求助? 2837794
关于积分的说明 8017206
捐赠科研通 2500478
什么是DOI,文献DOI怎么找? 1334942
科研通“疑难数据库(出版商)”最低求助积分说明 637363
邀请新用户注册赠送积分活动 605381