Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
4秒前
顾矜应助山茶采纳,获得10
5秒前
动听的薯条完成签到,获得积分10
5秒前
宋十一完成签到 ,获得积分10
6秒前
晶莹黎发布了新的文献求助10
11秒前
wangermazi完成签到,获得积分0
13秒前
听话的幼蓉完成签到,获得积分20
16秒前
longtengfei完成签到,获得积分10
19秒前
英喆完成签到 ,获得积分10
20秒前
小二郎应助jewelliang采纳,获得10
21秒前
大力水手完成签到,获得积分10
24秒前
星辰大海应助科研不通采纳,获得10
26秒前
晶莹黎完成签到,获得积分10
27秒前
27秒前
贪玩枫叶完成签到 ,获得积分10
28秒前
许七安发布了新的文献求助10
31秒前
ethan2801完成签到,获得积分10
31秒前
33秒前
pterionGao完成签到 ,获得积分10
34秒前
鹿雅彤发布了新的文献求助10
34秒前
海猫食堂完成签到,获得积分10
36秒前
jewelliang发布了新的文献求助10
38秒前
YQT完成签到 ,获得积分10
39秒前
wt完成签到,获得积分10
40秒前
桐桐应助Gao采纳,获得10
40秒前
Stars完成签到,获得积分10
41秒前
44秒前
jjlyy完成签到,获得积分10
45秒前
YTT完成签到,获得积分10
46秒前
47秒前
00完成签到 ,获得积分10
47秒前
actor2006完成签到,获得积分10
47秒前
JY'完成签到,获得积分0
49秒前
oyly完成签到 ,获得积分10
49秒前
幽默的溪灵给末末的求助进行了留言
49秒前
Wendy含发布了新的文献求助10
50秒前
张一二二二完成签到,获得积分10
51秒前
51秒前
Orange应助jewelliang采纳,获得10
51秒前
ZZ完成签到 ,获得积分10
52秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
Why I Chose China [by Morris R. Wills] in "Look", February 8 and 22, 1966; 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190169
求助须知:如何正确求助?哪些是违规求助? 2839481
关于积分的说明 8023899
捐赠科研通 2502370
什么是DOI,文献DOI怎么找? 1336458
科研通“疑难数据库(出版商)”最低求助积分说明 637841
邀请新用户注册赠送积分活动 606020