Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
彭于晏应助Zhu采纳,获得10
刚刚
希望天下0贩的0应助FG采纳,获得10
刚刚
cvg发布了新的文献求助10
1秒前
2秒前
paopao发布了新的文献求助10
3秒前
5秒前
6秒前
sg发布了新的文献求助10
8秒前
FG完成签到,获得积分10
9秒前
小小仙完成签到,获得积分10
9秒前
司空珩完成签到,获得积分10
9秒前
mengzhonghunli完成签到,获得积分10
10秒前
FG发布了新的文献求助10
12秒前
香蕉觅云应助幸福墨镜采纳,获得10
12秒前
爆米花应助和谐的棉花糖采纳,获得10
12秒前
12秒前
13秒前
czt完成签到 ,获得积分10
14秒前
小二郎应助微笑的鱼采纳,获得10
15秒前
科研通AI2S应助陌上疏采纳,获得10
19秒前
Owen应助嗯九采纳,获得10
19秒前
20秒前
sg完成签到,获得积分10
21秒前
23秒前
24秒前
25秒前
周周发布了新的文献求助10
26秒前
27秒前
hong发布了新的文献求助20
27秒前
28秒前
28秒前
zzk完成签到,获得积分10
29秒前
thomas发布了新的文献求助30
30秒前
youjiang发布了新的文献求助10
30秒前
30秒前
万事胜意完成签到,获得积分10
31秒前
32秒前
称心花卷发布了新的文献求助10
33秒前
33秒前
34秒前
高分求助中
Evolution 3rd edition 1500
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
Ribozymes and aptamers in the RNA world, and in synthetic biology 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3180854
求助须知:如何正确求助?哪些是违规求助? 2831074
关于积分的说明 7982996
捐赠科研通 2492997
什么是DOI,文献DOI怎么找? 1329955
科研通“疑难数据库(出版商)”最低求助积分说明 635850
版权声明 602954