Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
谢会会完成签到 ,获得积分10
2秒前
4秒前
ruiii完成签到 ,获得积分10
5秒前
科研通AI2S应助冕镜采纳,获得10
6秒前
ZhMeY发布了新的文献求助20
7秒前
wx1完成签到 ,获得积分0
12秒前
ybwei2008_163发布了新的文献求助10
14秒前
zhangzhangzhang完成签到 ,获得积分10
18秒前
俊俊完成签到 ,获得积分0
24秒前
楚襄谷完成签到 ,获得积分10
25秒前
Cynthia42完成签到 ,获得积分10
29秒前
jeffrey完成签到,获得积分10
29秒前
李健的粉丝团团长应助yuan采纳,获得10
33秒前
Ava应助ybwei2008_163采纳,获得10
39秒前
slp完成签到 ,获得积分10
54秒前
李爱国应助ybwei2008_163采纳,获得10
57秒前
apckkk完成签到 ,获得积分10
58秒前
ZhMeY完成签到,获得积分20
1分钟前
Gnaeus完成签到 ,获得积分10
1分钟前
纳米完成签到 ,获得积分10
1分钟前
遂安完成签到,获得积分10
1分钟前
昭荃完成签到 ,获得积分10
1分钟前
莓烦恼完成签到 ,获得积分10
1分钟前
正直的松鼠完成签到 ,获得积分10
1分钟前
tmobiusx完成签到,获得积分20
1分钟前
1分钟前
大将军完成签到,获得积分10
1分钟前
ybwei2008_163发布了新的文献求助10
1分钟前
劲秉应助科研通管家采纳,获得20
1分钟前
劲秉应助科研通管家采纳,获得10
1分钟前
Zjn-完成签到 ,获得积分10
1分钟前
阳炎完成签到,获得积分10
1分钟前
勤奋青寒完成签到,获得积分10
1分钟前
651完成签到 ,获得积分10
1分钟前
1分钟前
俏皮诺言完成签到 ,获得积分10
1分钟前
ybwei2008_163发布了新的文献求助10
1分钟前
jameslee04完成签到 ,获得积分10
2分钟前
03210322完成签到 ,获得积分10
2分钟前
修水县1个科研人完成签到 ,获得积分10
2分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190097
求助须知:如何正确求助?哪些是违规求助? 2839411
关于积分的说明 8023505
捐赠科研通 2502244
什么是DOI,文献DOI怎么找? 1336398
科研通“疑难数据库(出版商)”最低求助积分说明 637836
邀请新用户注册赠送积分活动 605974