Loganin alleviates myocardial ischemia–reperfusion injury through GLP‐1R/NLRP3‐mediated pyroptosis pathway

上睑下垂 罗格宁 细胞凋亡 化学 再灌注损伤 免疫印迹 药理学 污渍 程序性细胞死亡 缺血 生物 生物化学 医学 内科学 高效液相色谱法 色谱法 基因
作者
Wenfan Li,Pei Fan,Xiaobo Wang,Haifeng Tang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:38 (11): 2730-2740 被引量:3
标识
DOI:10.1002/tox.23908
摘要

Myocardial ischemia-reperfusion (I/R) injury is one of main pathological manifestations of cardiovascular outcomes related to NLRP3 inflammasome-mediated pyroptosis pathway. Loganin is an iridoid glycoside extracted from traditional Chinese medicines, which has multiple activities. However, the roles and mechanism of loganin in myocardial I/R injury remain largely unknown. The models of myocardial I/R injury were established using I/R-treated rats or OGD/R-treated H9C2 cardiomyocytes. Myocardial damage was assessed by TTC and hematoxylin-eosin staining. Pyroptosis-related marker levels were detected by immunohistochemistry, immunofluorescence and western blotting assays. Cell proliferation was examined via EdU assay. Cell apoptosis was investigated by LDH release and flow cytometry. The integrity of cell membrane was analyzed via Dil staining. GLP-1R and NLRP3 levels were detected by immunofluorescence and western blotting assays. Our results showed that loganin suppressed I/R-induced myocardial damage in rats by reducing myocardial infarct, injury and pyroptosis. In addition, loganin attenuated OGD/R-induced cardiomyocyte apoptosis through increasing cell proliferation and reducing LDH release and apoptotic rate. Loganin also mitigated OGD/R-induced cardiomyocyte pyroptosis by reducing cell membrane damage and levels of cleaved caspase-1, IL-1β and IL-18. Furthermore, loganin repressed GLP-1R/NLRP3 pathway activation in OGD/R-treated H9C2 cardiomyocytes by enhancing GLP-1R expression and decreasing NLRP3 level. GLP-1R/NLRP3 activation by GLP-1R inhibition or NLRP3 overexpression reversed the suppressive effects of loganin on OGD/R-induced cardiomyocyte pyroptosis. These data indicated that loganin prevented OGD/R-induced proliferation inhibition, apoptosis and pyroptosis in OGD/R-treated cardiomyocytes by inhibiting GLP-1R/NLRP3 activity, indicating the therapeutic potential of loganin in myocardial I/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
Lin琳发布了新的文献求助10
4秒前
pbc完成签到,获得积分10
5秒前
5秒前
lijing123完成签到,获得积分10
5秒前
辜月十二完成签到 ,获得积分10
5秒前
6秒前
冒险寻羊应助puzhongjiMiQ采纳,获得10
7秒前
冒险寻羊应助puzhongjiMiQ采纳,获得10
7秒前
冒险寻羊应助puzhongjiMiQ采纳,获得10
7秒前
冒险寻羊应助puzhongjiMiQ采纳,获得10
7秒前
冒险寻羊应助puzhongjiMiQ采纳,获得10
7秒前
冒险寻羊应助puzhongjiMiQ采纳,获得10
7秒前
7秒前
顾白凡应助puzhongjiMiQ采纳,获得10
7秒前
随机子应助puzhongjiMiQ采纳,获得10
7秒前
华仔应助puzhongjiMiQ采纳,获得10
7秒前
乐乐应助左左采纳,获得10
7秒前
珠手知由发布了新的文献求助10
8秒前
DDQHB发布了新的文献求助10
8秒前
9秒前
聪慧的从雪完成签到 ,获得积分10
10秒前
rr发布了新的文献求助10
11秒前
李健的小迷弟应助MMMM采纳,获得30
12秒前
梅一一完成签到,获得积分10
12秒前
14秒前
LU完成签到,获得积分10
15秒前
15秒前
大气伯云发布了新的文献求助10
16秒前
16秒前
汉堡包应助whq531608采纳,获得30
16秒前
DDQHB完成签到,获得积分10
17秒前
18秒前
18秒前
小灰灰完成签到 ,获得积分10
18秒前
bella发布了新的文献求助20
21秒前
21秒前
Return发布了新的文献求助10
22秒前
指尖心事完成签到,获得积分10
24秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190354
求助须知:如何正确求助?哪些是违规求助? 2839625
关于积分的说明 8024732
捐赠科研通 2502549
什么是DOI,文献DOI怎么找? 1336609
科研通“疑难数据库(出版商)”最低求助积分说明 637841
邀请新用户注册赠送积分活动 606051