Individual Variability of Human Cortical Spontaneous Activity by 3T/7T fMRI

Mapping variability in cortical spontaneous activity (CSA) is an essential goal of understanding various sources of dark brain energy in human neuroscience. CSA was traditionally characterized using resting-state functional MRI (rfMRI) at 1.5T or 3T magnets while recently with 7T-rfMRI. However, the utility and interpretability of 7T-rfMRI must first be established for its variability. By leveraging rfMRI data from the Human Connectome Project (HCP), we derived CSA metrics with 3T-rfMRI and 7T-rfMRI for the same 84 healthy participants (52 females). The 7T-rfMRI produces different CSA metrics at multiple spatial-scales and their variability from the 3T-rfMRI. These differences were spatially dependent and varied according to specific cortical organization. For the amplitude metric, 7T-rfMRI enhanced its spatial contrasts in the anterior cortex but weakened it in the posterior cortex. An opposite pattern was observed for the connectivity metrics. The reliability changes of these metrics were scale dependent, indicating enhanced reliability for connectivity but weakened reliability for amplitude by 7T-rfMRI. These effects were primarily located in the high-order associate cortex, parsing the corresponding changes in individual differences with respect to 7T-rfMRI: (1) higher connectivity variability between participants and the lower connectivity variability within individual participants, and (2) lower amplitude variability between participants and higher amplitude variability within participants. Our work, for the first time, demonstrated the variability of the human CSA across space, rfMRI settings/platforms, and individuals. We discussed the statistical implications of our findings on CSA-based experimental designs and reproducible neuroscience as well as their translational value for personalized applications.