细胞外小泡
微泡
小泡
计算生物学
RNA剪接
细胞生物学
环状RNA
选择性拼接
工作流程
外显子
核糖核酸
生物信息学
生物
小RNA
遗传学
计算机科学
膜
基因
数据库
作者
Jing Wang,Qiaojuan Li,Shenglin Huang
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 47-59
标识
DOI:10.1007/978-1-0716-3678-7_3
摘要
Extracellular vesicles (EVs) are small vesicles secreted by various cell types and are enriched in multiple body fluids. EVs containing RNA have the potential to modulate biological processes and are being investigated for their diagnostic and therapeutic applications. Circular RNAs (circRNAs), generated through back-splicing of exons, are enriched in EVs. Given their unique characteristics and diverse functions, EV-circRNAs are important players in disease pathology. This chapter describes a workflow for investigating the expression profile of EV-circRNAs, which includes EVs separation, library preparation, and bioinformatics analysis. This workflow can aid the investigation of EV-circRNAs and their potential role in disease pathology.
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