生物
非酒精性脂肪肝
斑马鱼
转录组
脂肪肝
免疫系统
细胞
电池类型
免疫
疾病
先天免疫系统
炎症
肝细胞
肝病
细胞生物学
基因
内科学
基因表达
免疫学
遗传学
生物化学
医学
体外
作者
Yingyi Huang,Takayuki Matsuki,Hongyan Wang,Jian-Yang Chen,Xianghui Zhang,Yubang Li,Yifang Lu,Zhongdian Dong,Kaiqiang Liu,Zhongduo Wang,Qian Wang,Guangyi Fan,Jun Zou,Shanshan Liu,Changwei Shao
标识
DOI:10.1016/j.fsi.2024.109428
摘要
Nonalcoholic fatty liver disease (NAFLD) is becoming the most common chronic liver disease in the world. Immunity is the major contributing factor in NAFLD; however, the interaction of immune cells and hepatocytes in disease progression has not been fully elucidated. As a popular species for studying NAFLD, zebrafish, whose liver is a complex immune system mediated by immune cells and non-immune cells in maintaining immune tolerance and homeostasis. Understanding the cellular composition and immune environment of zebrafish liver is of great significance for its application in NAFLD. Here, we established a liver atlas that consists of 10 cell types using single-cell RNA sequencing (scRNA-seq). By examining the heterogeneity of hepatocytes and analyzing the expression of NAFLD-associated genes in the specific cluster, we provide a potential target cell model to study NAFLD. Additionally, our analysis identified two subtypes of distinct resident macrophages with inflammatory and non-inflammatory functions and characterized the successive stepwise development of T cell subclusters in the liver. Importantly, we uncovered the possible regulation of macrophages and T cells on target cells of fatty liver by analyzing the cellular interaction between hepatocytes and immune cells. Our data provide valuable information for an in-depth study of immune cells targeting hepatocytes to regulate the immune balance in NAFLD.
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