Molecular characterization of a Single negative stranded RNA virus from the rice blast fungus Magnaporthe oryzae isolate NJ39

Abstract A novel negative-sense single-stranded RNA mycovirus, designated as " Magnaporthe oryzae mymonavirus 1" (MoMNV1), was identifed in the rice blast fungus Magnaporthe oryzae isolate NJ39. MoMNV1 has a single genomic RNA segment consisting of 10,515 nucleotides, which contains six open reading frames. The largest open reading frame contains 5837 bases and encodes RNA replicase. The six open reading frames have no overlap and are linearly arranged on the genome, but the spacing of the genes is small, with a maximum of 315 bases and a minimum of 80 bases. Genome comparison and phylogenetic analysis indicated that MoMNV1 is a new member of the genus Penicillimonavirus in the family Mymonaviridae .