清脆的
基因组编辑
基因组
工作流程
计算生物学
生物
选择(遗传算法)
计算机科学
基因分型
基因
遗传学
数据库
人工智能
基因型
作者
Tianzhen Liu,Xuening Zhang,Kai Li,Qi Yao,Dating Zhong,Qi Deng,Yuming Lu
标识
DOI:10.1016/j.copbio.2022.102875
摘要
As a powerful genome editing technology, CRISPR/Cas is revolutionizing both fundamental research and crop breeding, and has now evolved into large-scale editing tools that are efficient, simple, and programmable. With such CRISPR screening technologies, the numbers of genome-edited crops are rapidly increasing. Here, we describe the general workflow of a CRISPR screen in plants, including the selection of appropriate editors, genome-wide guide RNA design, pooled library construction, massive transformation, and high-throughput genotyping. We also discuss applications for the screening of candidate genes, the optimization of spatiotemporal expression, the evolution of protein activities, and the establishment of genome-wide libraries of knockout mutant. After considering the current challenges and limitations, we finally envision a virus-mediated strategy to improve CRISPR screens.
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