Abstract Argas ticks are primary parasites of birds with veterinary importance. Nevertheless, these ticks have received little attention regarding molecular identification studies. DNA barcoding is a powerful technique for identifying tick species besides traditional morphological identification. The present work is a first effort to divulge DNA sequences of Argas ( Persicargas ) arboreus from Egypt and worldwide. We used cytochrome c oxidase subunit I (COI) from A. arboreus infesting herons, and from the fowl tick Argas ( Persicargas ) persicus . Our results pointed out another success for the Folmer primers that are widely used in DNA barcoding, permitting the discrimination of morphologically similar A. arboreus and A. persicus .