The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes

(Cell Stem Cell 29, 795–809.e1–e11; May 5, 2022) In the originally published version of this manuscript, in the “Day 14-18...” segment of the “Differentiation of human pluripotent stem cells to hepatocytes-like cells” subsection within the STAR Methods, trace element A and trace element B should have had values of 500 μL instead of 500 mL. In the subsequent subsection, “Expansion of hPSC-hepatocytes in vitro and reaggregation to form spheroids,” there are two parts describing trace element A as having a value of 500 mL and two parts describing trace element B as having a value of 500 mL. All four parts should list the value at 500 μL. To address this, the authors have now changed “500 mL” in all the instances listed here to “500 μL”. Additionaly, in the "For DOX-induction experiments...“ segment of the “Differentiation of human pluripotent stem cells to hepatocytes-like cells” subsection within the STAR Methods, DOX should have had a final concentration of 2 µg/mL instead of 2 mg/mL. To address this, the authors have now changed “2 mg/mL” in the instance listed here to “2 µg/mL.” The authors apologize for the oversight and for any resulting confusion. The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytesMa et al.Cell Stem CellApril 21, 2022In BriefBy developing a 3D spheroid system for hPSC-hepatocytes differentiation and combining it with genomic approaches, Jaenisch and colleagues identified a role for THRB in regulating hPSC-hepatocytes maturation through chromatin-remodeling complex pBAF. The differentiated hPSC-hepatocytes engraft into non-damaged mouse liver and proliferate in vitro in response to liver regeneration signals. Full-Text PDF Open Archive