Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry

Oligonucleotides are used in a variety of molecular biology techniques, from next-generation sequencing to genetic testing. Maintaining the sequence fidelity of synthetic oligonucleotides is critical to their use. This chapter describes the steps of solid phase oligonucleotide synthesis and purification, which enables the synthesis of oligonucleotides with specific sequences and high purity.