Closely spaced modes commonly observed in long-span suspension bridges can greatly increase the difficulty of identifying and tracking modal parameters. Most existing studies generally focus on identifying the closely spaced modes and quantifying the uncertainties based on numerical and experimental models. Further research focusing on full-scale long-span bridges is still required. A case study on identifying the closely spaced modes of the Qixiashan Yangtze River Bridge, a long-span suspension bridge with a main span of 1 418 m, is conducted in this paper. The effectiveness of the generalized fast Bayesian fast Fourier transform (GFBFFT) method is verified by both the simulated and monitoring data. The results show that a larger coefficient of variation (COV) and higher uncertainty is typically contained in the closely spaced modes than the separated modes. Compared with the FDD and SSI methods, the GFBFFT method guarantees higher identification accuracy of modal parameters and can serve as a reliable tool to identify the closely spaced modes.