Rapid Microfluidics Prototyping Through Variotherm Desktop Injection Molding for Multiplex Diagnostics

微流控 造型(装饰) 材料科学 快速成型 多路复用 环介导等温扩增 纳米技术 计算机科学 化学 生物信息学 复合材料 生物化学 生物 DNA
作者
Gianmarco Domenico Suarez,Steevanson Bayer,Yuki Yu Kiu Tang,Domenick Arrigo Suarez,Peter Pak-Hang Cheung,Stefan Nagl
出处
期刊:Lab on a Chip [Royal Society of Chemistry]
卷期号:23 (17): 3850-3861
标识
DOI:10.1039/d3lc00391d
摘要

In this work, we demonstrate an inexpensive method of prototyping microfluidics using a desktop injection molding machine. A centrifugal microfluidic device with a novel central filling mechanism was developed to demonstrate the technique. We overcame the limitations of desktop machines in replicating microfluidic features by variotherm heating and cooling the mold between 50 °C and 110 °C within two minutes. Variotherm heating enabled good replication of microfeatures, with a coefficient of variation averaging only 3.6% attained for the measured widths of 100 μm wide molded channels. Using this methodology, we produced functional polystyrene centrifugal microfluidic chips, capable of aliquoting fluids into 5.0 μL reaction chambers with 97.5% accuracy. We performed allele-specific loop-mediated isothermal amplification (AS-LAMP) reactions for genotyping CYP2C19 alleles on these chips. Readouts were generated using optical pH sensors integrated onto chips, by drop-casting sensor precursor solutions into reaction chambers before final chip assembly. Positive reactions could be discerned by decreases in pH sensor fluorescence, thresholded against negative control reactions lacking the primers for nucleic acid amplification and with time-to-results averaging 38 minutes. Variotherm desktop injection molding can enable researchers to prototype microfluidic devices more cost-effectively, in an iterative fashion, due to reduced costs of smaller, in-house molds. Designs prototyped this way can be directly translated to mass production, enhancing their commercialization potential and positive impacts.
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