Identification of TMexCD-TOprJ-producing carbapenem-resistant Gram-negative bacteria from hospital sewage

替加环素 微生物学 生物 铜绿假单胞菌 质粒 细菌 革兰氏阴性菌 抗生素耐药性 假单胞菌 碳青霉烯 流出 抗生素 基因 大肠杆菌 遗传学
作者
Jie Zhu,Jingnan Lv,Zhichen Zhu,Tao Wang,Xiaofang Xie,Haifang Zhang,Liang Chen,Hong Du
出处
期刊:Drug Resistance Updates [Elsevier]
卷期号:70: 100989-100989 被引量:14
标识
DOI:10.1016/j.drup.2023.100989
摘要

Carbapenems and tigecycline are crucial antimicrobials for the treatment of gram-negative bacteria infections. Recently, a novel resistance-nodulation-division (RND) efflux pump gene cluster, tmexCD-toprJ, which confers resistance to tigecycline, has been discovered in animals and clinical isolates. It was reported that hospital sewage could act as a reservoir for gram-negative bacteria with high antimicrobial resistance genes. In this study, we analyzed 84 isolates of carbapenem-resistant gram-negative bacteria (CR-GNB) from hospital sewage, and identified five isolates of TMexCD-ToprJ-producing CR-GNB, including one Raoultella ornithinolytica isolate and four Pseudomonas spp. isolates. All these five isolates carried at least one carbapenem resistance gene and were resistant to multiple antibiotics. Multiple tmexCD-toprJ clusters were detected, including tmexC2D2-toprJ2, tmexC3D3-toprJ3, tmexC3.2D3.3-toprJ1b and tmexC3.2D3-toprJ1b. Among these clusters, the genetic construct of tmexC3.2D3-toprJ1b showed 2-fold higher minimum inhibitory concentration (MIC) of tigecycline than other three variants. In addition, it was found that the tmexCD-toprJ gene cluster was originated from Pseudomonas spp. and mainly located on Tn6855 variants inserted in the same umuC-like genes on chromosomes and plasmids. This unit co-localized with blaIMP or blaVIM on IncHI5-, IncpJBCL41- and IncpSTY-type plasmids in the five isolates of TMCR-GNB. The IncHI5- and IncpSTY-type plasmids had the ability to conjugal transfer to E. coli J53 and P. aeruginosa PAO1, highlighting the potential risk of transfer of tmexCD-toprJ from Pseudomonas spp. to Enterobacterales. Importantly, genomic analysis showed that similar tmexCD-toprJ-harboring IncHI5 plasmids were also detected in human samples, suggesting transmission between environmental and human sectors. The emergence of TMCR-GNB from hospital sewage underscores the need for ongoing surveillance of antimicrobial resistance genes, particularly the novel resistance genes such as the tmexCD-toprJ gene clusters in the wastewater environment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
hipig发布了新的文献求助10
8秒前
young完成签到 ,获得积分10
9秒前
leo完成签到,获得积分10
14秒前
Deanna完成签到 ,获得积分10
16秒前
18秒前
was_3完成签到,获得积分10
19秒前
要笑cc完成签到,获得积分10
20秒前
666完成签到 ,获得积分10
21秒前
binfo发布了新的文献求助10
22秒前
宣宣宣0733完成签到,获得积分10
22秒前
胡质斌完成签到,获得积分10
24秒前
深情安青应助科研通管家采纳,获得30
27秒前
吴晓娟完成签到 ,获得积分10
33秒前
隐形曼青应助hipig采纳,获得10
33秒前
xiang完成签到 ,获得积分10
36秒前
俊逸吐司完成签到 ,获得积分10
52秒前
落雪完成签到 ,获得积分10
58秒前
猪猪想要平静的生活完成签到 ,获得积分10
1分钟前
Wang发布了新的文献求助10
1分钟前
小喵完成签到 ,获得积分10
1分钟前
qiancib202完成签到,获得积分10
1分钟前
dwgwushan完成签到 ,获得积分10
1分钟前
Cold-Drink-Shop完成签到,获得积分10
1分钟前
香蕉觅云应助Wang采纳,获得10
1分钟前
1分钟前
流星雨发布了新的文献求助10
1分钟前
forest发布了新的文献求助10
1分钟前
Jeffery426完成签到,获得积分10
1分钟前
WY完成签到 ,获得积分10
1分钟前
baobeikk完成签到 ,获得积分10
1分钟前
我就想看看文献完成签到 ,获得积分10
1分钟前
Zhou发布了新的文献求助10
1分钟前
ylyao完成签到 ,获得积分10
1分钟前
林谷雨完成签到 ,获得积分10
2分钟前
不吃芹菜完成签到,获得积分10
2分钟前
陈炳蓉完成签到,获得积分10
2分钟前
xiaowuge完成签到 ,获得积分10
2分钟前
刘丰完成签到 ,获得积分10
2分钟前
现实的大白完成签到 ,获得积分10
2分钟前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Typology of Conditional Constructions 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3571332
求助须知:如何正确求助?哪些是违规求助? 3141926
关于积分的说明 9444874
捐赠科研通 2843331
什么是DOI,文献DOI怎么找? 1562830
邀请新用户注册赠送积分活动 731326
科研通“疑难数据库(出版商)”最低求助积分说明 718524