氨基糖苷
细菌
微生物学
化学
抗生素
生物
遗传学
作者
Chao Chen,Ying Li,Yilin Wang,Yu Sun,Yixuan Bao,Jianyu Zhang,Ruoyao Zhang,Ryan T. K. Kwok,Jacky W. Y. Lam,Duo Mao,Peng Hou,Ben Zhong Tang
标识
DOI:10.1002/adhm.202400362
摘要
The diminishing effectiveness of existing aminoglycoside antibiotics (AGs) compels us to seek new approaches to enhance the sensitivity of current AGs. Despite ongoing efforts, currently available approaches remain restricted. Herein, we introduce a novel strategy involving the rational construction of an aggregation-induced-emission luminogen (AIEgen) to significantly enhance Gram-positive bacteria's susceptibility to AGs. The application of this approach involves the simple addition of AIEgens to bacteria followed by a 5-minute light irradiation. Under light exposure, AIEgens efficiently generate ROS, elevating intrabacterial ROS levels to a non-lethal threshold. Post-treatment, the bacteria swiftly enter a hypersensitive state, resulting in a 21.9-fold, 15.5-fold, and 7.2-fold increase in susceptibility to three AGs: kanamycin, gentamycin, and neomycin, respectively. Remarkably, this approach is specific to AGs, and the induced hypersensitivity displays unparalleled longevity and heritability. Further in vivo studies confirm a 7.0-fold enhanced bactericidal ability of AGs against Gram-positive bacteria through this novel approach. This research not only broadens the potential applications of AIEgens but also introduces a novel avenue to bolster the effectiveness of AGs in combatting bacterial infections. This article is protected by copyright. All rights reserved.
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