Microsphere lens array embedded microfluidic chip for SERS detection with simultaneous enhancement of sensitivity and stability

微流控 灵敏度(控制系统) 镜头(地质) 材料科学 炸薯条 微流控芯片 实验室晶片 微球 纳米技术 光学 计算机科学 电子工程 工程类 物理 电信 化学工程
作者
Zhenyong Dong,Xiaoxian Liu,Song Zhou,Yifan Zhu,Jin Chen,Yukai Liu,Xiao Ming Ren,Yanqing Lu,Rui Xiao,Guanghui Wang
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:261: 116505-116505 被引量:4
标识
DOI:10.1016/j.bios.2024.116505
摘要

Surface enhanced Raman spectroscopy (SERS) utilizes the fingerprint features of molecular vibrations to identify and detect substances. However, in traditional single focus excitation scenarios, its signal collection efficiency of the objective is restricted. Furthermore, the uneven distribution of samples on the SERS substrate would result in poor signal stability, while the excitation power is limited to avoid sample damage. SERS detection system always requires precise adjustment of focal length and spot size, making it difficult for point-of-care testing applications. Here, we proposed a SERS microfluidic chip with barium titanate microspheres array (BTMA) embedded using vacuum self-assembled hot-pressing method for SERS detection with simultaneous enhancement of sensitivity and stability. Due to photonic nano-jets and directional antenna effects, high index microspheres are perfect micro-lens for effective light focusing and signal collecting. The BTMA can not only disperse excitation beam into an array of focal points covering the target uniformly with very low signal fluctuation, but enlarge the power threshold for higher signal intensity. We conducted a proof-of-principle experiment on chip for the detection of bacteria with immuno-magnetic tags and immuno-SERS tags. Together with magnetic and ultrasonic operations, the target bacteria in the flow were evenly congregated on the focal plane of BTMA. It demonstrated a limit of detection of 5 cells/mL, excellent signal reproducibility (error∼4.84%), and excellent position tolerance of 500 μm in X-Y plane (error∼5.375%). It can be seen that BTMA-SERS microfluidic chip can effectively solve the contradiction between sensitivity and stability in SERS detection.
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