灰葡萄孢菌
烟草花叶病毒
槐花
超氧化物歧化酶
菌丝体
化学
生物
多酚
活性氧
多酚氧化酶
生物化学
过氧化物酶
植物
抗氧化剂
酶
医学
病毒
替代医学
中医药
病理
病毒学
作者
Zhan‐Xing Hu,Ji Zhang,Tong Zhang,Cai-Yan Tian,Qiao An,Ping Yi,Chun‐Mao Yuan,Zhongkai Zhang,Lihua Zhao,Xiao‐Jiang Hao
标识
DOI:10.1021/acs.jafc.4c00992
摘要
As a continuous flow investigation of novel pesticides from natural quinolizidine alkaloids, the chemical compositions of the seeds of Sophora alopecuroides were thoroughly researched. Fifteen new aloperine-type alkaloids (1–15) as well as six known aloperine-type alkaloids (16–21) were obtained from the extract of S. alopecuroides. The structures of 1–21 were confirmed via HRESIMS, NMR, UV, IR, ECD calculations, and X-ray diffraction. The antiviral activities of 1–21 against tobacco mosaic virus (TMV) were detected following the improved method of half-leaf. Compared with ningnanmycin (protective: 69.7% and curative: 64.3%), 15 exhibited excellent protective (71.7%) and curative (64.6%) activities against TMV. Further biological studies illustrated that 15 significantly inhibited the transcription of the TMV-CP gene and increased the activities of polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL). The antifungal activities of 1–21 against Phytophythora capsica, Botrytis cinerea, Alternaria alternata, and Gibberella zeae were screened according to a mycelial inhibition test. Compound 13 displayed excellent antifungal activity against B. cinerea (EC50: 7.38 μg/mL). Moreover, in vitro antifungal mechanism studies displayed that 13 causes accumulation of reactive oxygen species and finally leads to mycelia cell membrane damage and cell death in vitro.
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