足细胞
突触素
波多辛
嘌呤霉素
蛋白尿
尼福林
药理学
肾病综合征
化学
细胞生物学
肾
医学
生物
内科学
生物化学
蛋白质生物合成
作者
Xiujin Shen,Hong Jiang,Meike Ying,Zhoutao Xie,Xiayu Li,Haibing Wang,Jie Zhao,Chuan Lin,Yucheng Wang,Shi Feng,Jia Shen,Chunhua Weng,Weiqiang Lin,Huiping Wang,Qin Zhou,Yan Bi,Meng Li,Lingyan Wang,Tongyu Zhu,Xiao‐Ru Huang,Hui‐Yao Lan,Jing Zhou,Jianghua Chen
摘要
Podocyte injury and the appearance of proteinuria are features of minimal-change disease (MCD). Cyclosporin A (CsA) and tacrolimus (FK506) has been reported to reduce proteinuria in patients with nephrotic syndrome, but mechanisms remain unknown. We, therefore, investigated the protective mechanisms of CsA and FK506 on proteinuria in a rat model of MCD induced by puromycin aminonucleoside (PAN) and in vitro cultured mouse podocytes. Our results showed that CsA and FK506 treatment decreased proteinuria via a mechanism associated to a reduction in the foot-process fusion and desmin, and a recovery of synaptopodin and podocin. In PAN-treated mouse podocytes, pre-incubation with CsA and FK506 restored the distribution of the actin cytoskeleton, increased the expression of synaptopodin and podocin, improved podocyte viability, and reduced the migrating activities of podocytes. Treatment with CsA and FK506 also inhibited PAN-induced podocytes apoptosis, which was associated with the induction of Bcl-xL and inhibition of Bax, cleaved caspase 3, and cleaved PARP expression. Further studies revealed that CsA and FK506 inhibited PAN-induced p38 and JNK signaling, thereby protecting podocytes from PAN-induced injury. In conclusion, CsA and FK506 inhibit proteinuria by protecting against PAN-induced podocyte injury, which may be associated with inhibition of the MAPK signaling pathway.
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