轨道轨道
质谱法
碎片(计算)
化学
混合质谱仪
色谱法
肽
蛋白质组学
串联质谱法
分析化学(期刊)
计算机科学
选择性反应监测
生物化学
基因
操作系统
作者
Guadalupe Espadas,Eva Borràs,Cristina Chiva,Eduard Sabidó
出处
期刊:Proteomics
[Wiley]
日期:2017-03-07
卷期号:17 (9)
被引量:43
标识
DOI:10.1002/pmic.201600416
摘要
One of the major additions in MS technology has been the irruption of the Orbitrap mass analyzer, which has boosted the proteomics analyses of biological complex samples since its introduction. Here, we took advantage of the capabilities of the new Orbitrap Fusion Lumos Tribrid mass spectrometer to assess the performance of different data‐dependent acquisition methods for the identification and quantitation of peptides and phosphopeptides in single‐shot analysis of human whole cell lysates. Our study explored the capabilities of tri‐hibrid mass spectrometers for (phospho‐) peptide identification and quantitation using different gradient lengths, sample amounts, and combinations of different peptide fragmentation types and mass analyzers. Moreover, the acquisition of the same complex sample with different acquisition methods resulted in the generation of a dataset to be used as a reference for further analyses, and a starting point for future optimizations in particular applications.
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