Attitude stabilization control via disturbance observer for flexible spacecraft is studied in this paper. First, a model based on Takagi-Sugeno fuzzy system of flexible spacecraft is established. In the case of spacecraft subjected to the external disturbance, the external disturbance and flexible accessories are treated as one disturbance. Furthermore, the lumped disturbance is no longer limited by bounded or zero-order derivatives. Then, a disturbance observer is proposed to estimate it while the gains are determined by the convergence condition of the estimation error. According to the obtained estimate, an integral sliding surface is selected so that the control law is partially composed of PDCC. At last, the effectiveness of the disturbance observer and attitude control law are illustrated by an example of a flexible spacecraft.