F-box protein At1g08710 negatively regulates root length and imparts drought stress tolerance inArabidopsis thaliana

F盒蛋白 Skp1型 拟南芥 泛素连接酶 生物 细胞生物学 拟南芥 蛋白质降解 泛素 非生物胁迫 卡林 基因 突变体 遗传学
作者
Venkateswara Rao,Virupapuram Vijaybhaskar
标识
DOI:10.1101/2020.08.31.275271
摘要

Abstract Plants experience abiotic stresses throughout their life cycle and accordingly respond to tide over the unfavorable conditions. Drought or water deficit is one such condition to which plants respond in various ways including the ubiquitin proteasome system (UPS). Ubiquitin E3 ligases are a diverse family of protein complexes of which Skp1□Cullin□F□box (SCF) class mediate the ubiquitination and subsequent proteolytic turnover of proteins. F□box protein subunit plays crucial role in imparting specificity for selective degradation of target proteins. Here we report the function of Arabidopsis F-box protein At1g08710 in drought stress adaptation. As F-box protein is a constituent of SCF complex, here it is shown interacting with ASK1 and Cullin1. F-box protein localizes to both nucleus and membrane. F-box gene transcript accumulates highly in root and is altered in response to drought stress conditions. F-box protein interacts with a transcriptional co-activator protein ADA2b. F-box mutant plants displayed better growth under drought stress conditions compared to the wild type with a reduced accumulation of H 2 O 2 and malondialdehyde (MDA). Drought responsive genes RD29A, RD22, ABI3 expression is also induced in F-box mutant plants. These results indicate F-box protein At1g08710 plays a role in drought stress adaptation in Arabidopsis thaliana . Highlights F-box gene At1g08710 encodes a nuclear, membrane localized protein. F-box protein At1g08710 interacts with transcriptional co-activator protein ADA2b. F-box protein imparts drought stress tolerance by modulating H 2 O 2 and MDA content. Drought response genes RD29A, RD22, ABI3 expression is induced in F-box mutant plants.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
玥来玥好发布了新的文献求助10
1秒前
沙拉依丁完成签到,获得积分10
1秒前
壮观以松发布了新的文献求助10
2秒前
2秒前
zc发布了新的文献求助10
3秒前
美丽的向雁完成签到,获得积分10
3秒前
4秒前
tiko完成签到,获得积分10
8秒前
李爱国应助三度和弦采纳,获得10
9秒前
冷静的荣轩完成签到,获得积分20
9秒前
9秒前
秋秋完成签到,获得积分10
10秒前
洪艳发布了新的文献求助10
10秒前
10秒前
由怜雪完成签到,获得积分10
10秒前
wyh完成签到,获得积分10
10秒前
RLL完成签到,获得积分10
11秒前
三叶完成签到,获得积分10
11秒前
斯文败类应助ewetylgkhlj采纳,获得10
12秒前
科目三应助Jing采纳,获得10
13秒前
13秒前
幸福大碗完成签到,获得积分10
13秒前
14秒前
张萌发布了新的文献求助10
14秒前
鹿静枫发布了新的文献求助10
15秒前
orixero应助三叶采纳,获得10
16秒前
饼子完成签到 ,获得积分10
17秒前
17秒前
大个应助咕咕采纳,获得10
18秒前
万能图书馆应助枝兮旅人采纳,获得10
18秒前
dinaa发布了新的文献求助10
18秒前
Akim应助纸飞机采纳,获得10
18秒前
星辰大海应助misalia采纳,获得10
19秒前
20秒前
leeyc发布了新的文献求助10
20秒前
牛大壮完成签到,获得积分10
20秒前
22秒前
魔幻的醉柳完成签到,获得积分10
23秒前
23秒前
hhh完成签到 ,获得积分10
23秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
Teaching language in context (Third edition) by Derewianka, Beverly; Jones, Pauline 550
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3581613
求助须知:如何正确求助?哪些是违规求助? 3151206
关于积分的说明 9486567
捐赠科研通 2853141
什么是DOI,文献DOI怎么找? 1568469
邀请新用户注册赠送积分活动 734700
科研通“疑难数据库(出版商)”最低求助积分说明 720769