Astragalus membranaceus and Salvia miltiorrhiza ameliorates cyclosporin A-induced chronic nephrotoxicity through the “gut-kidney axis”

丹参 肾脏疾病 医学 肾毒性 小桶 黄芪 肾功能 药理学 生物 内科学 中医药 传统医学 内分泌学 基因表达 病理 基因 生物化学 转录组 替代医学
作者
Cong Han,Yuehua Jiang,Wei Li,Yao Liu
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:269: 113768-113768 被引量:47
标识
DOI:10.1016/j.jep.2020.113768
摘要

The combination of Astragalus membranaceus and Salvia miltiorrhiza (AS) is an effective prescription that is widely used to treat chronic kidney disease (CKD) clinically in traditional Chinese medicine. Our previous studies have shown that AS can alleviate early CKD through the “gut-kidney axis”, but the regulatory role of AS in the “gut-kidney axis” in the middle and late stages of CKD caused by cyclosporin A-induced chronic nephrotoxicity (CICN) has remained unclear. To explore the protective effect of AS by regulating the intestinal flora to further control the miRNA-mRNA interaction profiles in CICN. Thirty-two mice were divided into four groups: Normal (N) (olive oil), Model (M) (CsA, 30 mg kg−1 d−1), AS (CsA + AS, 30 + 8.4 g kg−1 d−1) and FMT-AS (CsA + Faeces of AS group, 30 mg + 10 mL kg−1 d−1). The mice were treated for 6 weeks. Changes in renal function related metabolites were detected, pathological changes in the colon and kidney were observed, and 16S rDNA sequencing was performed on mouse faeces. In addition, miRNA and mRNA sequencing were performed on the kidney to construct differential expression (DE) profiles of the other 3 groups compared with group M. The target mRNAs among the DE miRNAs were then predicted, and an integrated analysis was performed with the DE mRNAs to annotate gene function by KEGG. DE miRNAs and DE mRNAs related to CICN in the overlapping top 20 KEGG pathways were screened and verified. Eight metabolites that could worsen renal function were increased in group M, accompanied by thickening of the glomerular basement membrane, vacuolar degeneration of renal tubules, and proliferation of collagen fibres, while AS and FMT-AS intervention amended these changes to varying degrees. Simultaneously, intestinal permeability increased, the abundance and diversity of the flora decreased, and the ratio of Firmicum to Bacteroides (F/B) increased in group M. The AS and FMT-AS treatments reversed the flora disorder and increased probiotics producing butyric acid and lactic acid, especially Akkermansia and Lactobacillus, which might regulate the 12 overlapping top 20 KEGG pathways, such as Butanoate metabolism, Tryptophan metabolism and several RF-related pathways, leading to the remission of renal metabolism. Finally, 15 DE miRNAs and 45 DE mRNAs were screened as the therapeutic targets, and the results coincided with the sequencing results. AS could alleviate renal fibrosis and metabolism caused by CICN through the “gut-kidney axis”. Probiotics such as Akkermansia and Lactobacillus were the primary driving factors, and the miRNA-mRNA interaction profiles, especially Butanoate metabolism and Tryptophan metabolism, may be an important subsequent response and regulatory mechanism.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
bbczj发布了新的文献求助10
刚刚
1秒前
2秒前
南风知我意完成签到,获得积分20
3秒前
段一帆发布了新的文献求助30
5秒前
wangqinlei完成签到 ,获得积分10
5秒前
fenghp发布了新的文献求助10
6秒前
王馨雨发布了新的文献求助10
6秒前
8秒前
CipherSage应助ccalvintan采纳,获得10
9秒前
9秒前
雪天的阳完成签到 ,获得积分10
11秒前
12秒前
13秒前
13秒前
烟花应助ren采纳,获得10
14秒前
讨厌科研发布了新的文献求助10
14秒前
量子星尘发布了新的文献求助10
15秒前
苏卿应助科研通管家采纳,获得30
16秒前
fd163c应助科研通管家采纳,获得10
17秒前
香蕉觅云应助科研通管家采纳,获得10
17秒前
思源应助科研通管家采纳,获得10
17秒前
SYLH应助科研通管家采纳,获得10
17秒前
17秒前
CAOHOU应助科研通管家采纳,获得10
17秒前
爆米花应助科研通管家采纳,获得10
17秒前
SYLH应助科研通管家采纳,获得30
17秒前
小蘑菇应助科研通管家采纳,获得10
17秒前
17秒前
殷勤的紫槐完成签到,获得积分10
17秒前
风轻青柠发布了新的文献求助10
18秒前
18秒前
机智冬灵完成签到,获得积分10
19秒前
20秒前
为小嗳打伞完成签到 ,获得积分10
22秒前
小木安华发布了新的文献求助10
22秒前
体贴的之卉完成签到,获得积分20
24秒前
大侠完成签到 ,获得积分10
24秒前
spy777应助机智冬灵采纳,获得20
25秒前
晨曦发布了新的文献求助10
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989390
求助须知:如何正确求助?哪些是违规求助? 3531487
关于积分的说明 11254109
捐赠科研通 3270153
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809174