荧光
荧光寿命成像显微镜
光漂白
双光子激发显微术
生命科学中的荧光
生物成像
临床前影像学
材料科学
纳米技术
分子成像
费斯特共振能量转移
光学
体内
物理
生物
生物技术
作者
Qing Lu,Chengjuan Wu,Zhiqiang Liu,Guangle Niu,Xiaoqiang Yu
标识
DOI:10.3389/fchem.2020.617463
摘要
Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.
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