天冬氨酸转氨酶
黑色素
化学
白细胞介素
肿瘤坏死因子α
丙氨酸转氨酶
药理学
细胞因子
生物化学
酶
生物
免疫学
内科学
医学
碱性磷酸酶
作者
Can Xu,Tingting Chen,Jinglei Li,Mingzhi Jin,Ming Ye
标识
DOI:10.1016/j.procbio.2019.08.022
摘要
In the present study, an intracellular melanin (LIM) isolated from Lachnum YM30 was obtained by submerged fermentation, extraction and purification. The possible structural formula of LIM was concluded, based on Ultraviolet (UV) analysis, IR analysis, elemental analysis, Nuclear Magnetic Resonance (NMR) and ESI-MS analysis, which showed the typical feature of fungal eumelanin. The hepatoprotective effect of LIM was evaluated in an acute LPS/D-GalN induced liver injury mouse model that was proved by the declining of alanine aminotransferase (ALT) and aspartate transaminase (AST), enhancing the content of anti-inflammatory factors interleukin-4 (IL-4) and interleukin-10 (IL-10). Concurrently, LIM could alleviate LPS/D-GalN toxicity through the effects of NF-kappa B (NF-κB) signaling pathway and its related cytokines interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α) and interleukin-8 (IL-8). The mechanisms underlying the protective effect might be related to inhibition of nuclear factor-kappa B activation and the functional groups of LIM. And compared with the single positive drug treatment or the melanin, the results showed that the combined treatment group could further reduce the inflammatory reaction of the model mice.
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