A novel signal-off photoelectrochemical biosensor for M.SssI MTase activity assay based on GQDs@ZIF-8 polyhedra as signal quencher

信号(编程语言) 化学 多面体 生物传感器 纳米技术 材料科学 分析化学(期刊) 色谱法 计算机科学 数学 几何学 程序设计语言
作者
Leixia Meng,Ke Xiao,Xiaohua Zhang,Cuicui Du,Jinhua Chen
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:150: 111861-111861 被引量:65
标识
DOI:10.1016/j.bios.2019.111861
摘要

DNA methylation catalyzed by M.SssI methyltransferases (MTase) has important roles in gene expression and other cellular activities, and relates to some diseases, especially cancers. Therefore, it is important to develop a sensitive sensing platform for M.SssI MTase activity assay. Here, taking zeolitic imidazolate framework-8 (ZIF-8) polyhedra as the carriers of graphene quantum dots (GQDs), GQDs-embedded ZIF-8 polyhedra (denoted as [email protected] polyhedra) were successfully prepared and used as the multi-functional signal quencher to construct a novel signal-off photoelectrochemical (PEC) biosensor for M.SssI MTase activity assay. Firstly, the indium tin oxide (ITO) slice was modified with TiO2, poly(diallyldimethylammonium chloride) and CdTe quantum dots (QDs). The obtained electrode was used as the photoelectrode and labeled as ITO/TiO2/CdTe QDs. Then, single-stranded DNA (S1) was anchored on the photoelectrode surface via S–Cd bond. After hybridization between S1 and biotinylated single-stranded DNA (S2), the streptavidin (SA)-labeled [email protected] polyhedra were introduced to the modified electrode via the specific reaction between biotin and SA. As the signal quencher, [email protected] polyhedra could not only inhibit the photocurrent signal of the ITO/TiO2/CdTe QDs electrode due to the steric hindrance effect, but also act as peroxidase mimetics to catalyze precipitation reaction of 4-chloro-1-naphthol, resulting in the evident depression of the photocurrent signal. For the specially designed S1/S2 double-strand DNA, the decreased photocurrent was quantitatively correlated with the M.SssI MTase activity (linear response range, 0.005–150 U mL−1; detection limit, 0.004 U mL−1). The developed [email protected] polyhedra and related PEC biosensor may have potential applications in clinical research and disease diagnosis.
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