内生
生物
竞争性内源性RNA
基因
引导RNA
长非编码RNA
细胞生物学
抄写(语言学)
Cas9
核糖核酸
基因表达
清脆的
计算生物学
遗传学
转录因子
生物化学
语言学
哲学
作者
Ni Gao,Jing Hu,Bingbing He,Zhengbang Ji,Xin Hu,Jia Jia Huang,Yu Wang,Jianpeng Peng,Yinghui Wei,Yingsi Zhou,Xiaowen Shen,Li He,Feng Xue,Qingquan Xiao,Linqi Shi,Yidi Sun,Chunmei Zhou,Haibo Zhou,Hui Yang
标识
DOI:10.1038/s41556-020-00610-9
摘要
Detection of endogenous signals and precise control of genetic circuits in the natural context are essential to understand biological processes. However, the tools to process endogenous information are limited. Here we developed a generalizable endogenous transcription-gated switch that releases single-guide RNAs in the presence of an endogenous promoter. When the endogenous transcription-gated switch is coupled with the highly sensitive CRISPR-activator-associated reporter we developed, we can reliably detect the activity of endogenous genes, including genes with very low expression (<0.001 relative to Gapdh; quantitative-PCR analysis). Notably, we could also monitor the transcriptional activity of typically long non-coding RNAs expressed at low levels in living cells using this approach. Together, our method provides a powerful platform to sense the activity of endogenous genetic elements underlying cellular functions.
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