外周血单个核细胞
流式细胞术
浅黄色外套
分离(微生物学)
染色
磷酸盐缓冲盐水
分子生物学
化学
细胞生长
细胞
色谱法
生物
免疫学
生物化学
微生物学
体外
遗传学
作者
Marco Cosentino,Elisa Storelli,Alessandra Luini,Massimiliano LM Legnaro,Emanuela Rasini,Marco Ferrari,Franca Marino
标识
DOI:10.17504/protocols.io.biahkab6
摘要
This recepe is used in the following protocols: - Separation and purification of human PBMC from FRESH BLOOD - Separation and purification of human PBMC from BUFFY COAT - Magnetic bead-based CD4+ T cell isolation from PBMCs with Dynabeads: CD4 Positive Isolation Kit - Magnetic bead-based TREG-TEFF cell isolation from PBMC with Miltenyi CD4+CD25+ Regulatory T cell Isolation Kit - Staining of human PBMC or ISOLATED SUBSETS with Cell Proliferation Dye-eFluor™ 670 (CPD-eFluor670) for cell proliferation evaluation by Flow Cytometry
科研通智能强力驱动
Strongly Powered by AbleSci AI