The study on neutralizing antibody detection with H1N1 subtype pseudotyped influenza viral particles

Objective To evaluate the effectiveness of pseudotyped influenza viral particles (pp) in neutralizing antibody (nAb) detection. Methods Pseudotyped particles of A(H1N1)pmd09 (A/California/7/2009) were generated. Pp in nAb detection were compared concurrently with the corresponding viruse by a hemagglutination inhibition test, as well as cytopathic effect- (CPE) and fluorescence-based neutralization assays. Results No significant difference between pp and the corresponding virus was observed in nAb detection of anti-H1N1 monoclonal antibody, NIBSC antiserum and goat immune serum by two assays (P>0.05). Moreover, pp and its corresponding virus had significant positive correlation (P<0.001), with the correlation coefficient near 1 in population sera detection of 300 samples collected in 2013. The positive rate of H1N1 antibody was 54.7% in population sera detection, and the geometric mean titers (95% confidence interval) [GMT (95%CI)] was 25.70 (22.39, 29.51). Conclusion Pp was reliable in neutralizing antibody detection. Key words: Orthomy xoviridae; Neutralization tests