双吖丙啶
点击化学
化学
鉴定(生物学)
组合化学
生物化学
生物物理学
生物
植物
作者
Andrew L. MacKinnon,Jack Taunton
标识
DOI:10.1002/9780470559277.ch090167
摘要
Target identification of biologically active small-molecules is often the rate-determining step in forward chemical genetics. Photo-affinity labeling (PAL) represents a useful biochemical strategy for target identification in complex protein mixtures. This unit describes the use of alkyl diazirine-based photo-affinity probes and Cu(I)-catalyzed click chemistry to covalently label and visualize the targets of biologically active small-molecules. A general method for affinity purification of probe-modified proteins, useful for identification of protein targets, is also described.
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