Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

AndrologiaVolume 54, Issue 8 e14486 ORIGINAL ARTICLE Beneficial effect of L-Proline supplementation on the quality of human spermatozoa Mojtaba Moradi, Mojtaba Moradi Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, IranSearch for more papers by this authorBahareh Moradi, Bahareh Moradi Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorAmir Hossein Hashemian, Amir Hossein Hashemian Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Biostatistics, School of Health, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorMitra Bakhtiari, Mitra Bakhtiari Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorMozafar Khazaei, Mozafar Khazaei orcid.org/0000-0003-0402-3333 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorFarzaneh Esmaeili, Farzaneh Esmaeili Infertility Treatment Research Center, Moatazedi Hospital, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorFaranak Aghaz, Faranak Aghaz Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorAzita Faramarzi, Corresponding Author Azita Faramarzi a.faramarzi90@gmail.com orcid.org/0000-0002-3779-4153 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Correspondence Azita Faramarzi, Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Email: a.faramarzi90@gmail.comSearch for more papers by this author Mojtaba Moradi, Mojtaba Moradi Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, IranSearch for more papers by this authorBahareh Moradi, Bahareh Moradi Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorAmir Hossein Hashemian, Amir Hossein Hashemian Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Biostatistics, School of Health, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorMitra Bakhtiari, Mitra Bakhtiari Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorMozafar Khazaei, Mozafar Khazaei orcid.org/0000-0003-0402-3333 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorFarzaneh Esmaeili, Farzaneh Esmaeili Infertility Treatment Research Center, Moatazedi Hospital, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorFaranak Aghaz, Faranak Aghaz Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranSearch for more papers by this authorAzita Faramarzi, Corresponding Author Azita Faramarzi a.faramarzi90@gmail.com orcid.org/0000-0002-3779-4153 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Correspondence Azita Faramarzi, Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Email: a.faramarzi90@gmail.comSearch for more papers by this author First published: 18 June 2022 https://doi.org/10.1111/and.14486Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract L-Proline is a natural anti-oxidative and osmoprotectant agent, playing a versatile role in cell metabolism and physiology. The present study aimed to explore the antioxidant effects of L-Proline on human sperm function during incubation. Thirty healthy, normozoospermic men (27–40 years) were enrolled. Sperm samples were incubated in an unsupplemented sperm medium (control group), or supplemented with L-Proline (1, 2 and 4 mmol/L) to evaluate its effect during 0, 1, 4 and 24 h of incubation. Sperm were assessed in terms of motility, viability, morphology, chromatin and DNA integrity. Moreover, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined in the sperm medium. The results indicated that 2 mmol/L of L-Proline significantly improved the maintenance of sperm motility, viability, normal morphology, chromatin and DNA integrity, and TAC levels compared to the control group during 24 h of incubation (p < 0.05). However, 1 and 4 mmol/L of L-Proline could not significantly preserve sperm parameters, chromatin quality, and antioxidant status during different incubation times compared to the control group (p > 0.05). Collectively, the inclusion of L-Proline (2 mmol/L) in the human sperm medium maintains sperm parameters and chromatin quality probably by modulating the oxidative status. CONFLICT OF INTEREST The authors declare no conflict of interest. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions. Volume54, Issue8September 2022e14486 RelatedInformation