Design of composite nanosupports and applications thereof in enzyme immobilization: A review

Enzyme immobilization techniques have developed dramatically over the past several decades. Support materials are key in shaping the function of a specific immobilized enzyme. Although they have large specific surface areas and functional active sites, single-component nanomaterials and their surface chemical modification derivatives struggle to meet increasing demand. Thus, composite materials, compounds of two or more materials, have been developed and applied in efficient immobilization through advances in materials science. More methods have been developed and employed to design composite nanomaterials in recent years. These novel composite nanomaterials often show superior physical, chemical, and biological performance as supports in enzyme immobilization, among other applications. In this review, immobilization techniques and their supports are stated first and methods to design and fabricate composite nanomaterials as nanosupports are also shown in the following section. Applications of composite nanosupports in laccase immobilization are discussed as models in the later sections of the paper. This review is intended to help readers gain insight into the design principles of composite nanomaterials for immobilization supports.