亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Identification of Candidate Gene Signatures and Regulatory Networks in Endometriosis and its Related Infertility by Integrated Analysis

子宫内膜异位症 生物 候选基因 基因 基因调控网络 不育 计算生物学 小RNA 生物信息学 基因表达 遗传学 医学 病理 怀孕
作者
Qiutong Li,Min Xi,Fangrong Shen,Fengqing Fu,Juan Wang,Youguo Chen,Jinhua Zhou
出处
期刊:Reproductive Sciences [Springer Nature]
卷期号:29 (2): 411-426 被引量:4
标识
DOI:10.1007/s43032-021-00766-1
摘要

Endometriosis is a common gynecological disease associated with infertility, and it represents an economic burden worldwide. However, the molecular mechanisms underlying endometriosis development have not yet been fully elucidated. Here, we aimed to identify reliable key genes and the related regulatory network that may be involved in endometriosis. Differentially expressed genes (DEGs) were identified through integrated analysis of four expression datasets of endometriosis from Gene Expression Omnibus. Gene functional analysis and protein-protein interaction network construction were performed to reveal the potential function of DEGs. Subsequently, candidate hub genes were defined and validated in GSE105764 dataset, and the associated regulatory networks were constructed. Additionally, GSE120103 dataset was applied to identify the differential expression between the infertile and fertile groups of patients with stage IV endometriosis. Finally, real-time quantitative polymerase chain reaction analysis was performed to identify the differential expression of hub genes in the collected clinical specimens. Robust rank aggregation integrated analysis determined 158 DEGs. Epithelial cell differentiation was the most significantly enriched biological process, and leukocyte transendothelial migration was the most significantly enriched pathway. Eight hub genes including CLDN3, CLDN5, CLDN7, CLDN11, HOXC8, HOXC6, HOXB6, and HOXB7 were identified, and most of these were validated as abnormally expressed genes in both the infertile group and patients with endometriosis. Transcriptional factors and microRNAs related to these genes were identified. Altogether, our integrated analysis identified critical gene signatures, involved pathways, and regulatory networks, which could provide clinically significant insights into the molecular mechanisms underlying endometriosis and its related infertility.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
YJL完成签到 ,获得积分10
1秒前
rrrred发布了新的文献求助10
5秒前
回眸完成签到 ,获得积分10
6秒前
Duduk完成签到 ,获得积分10
7秒前
8秒前
rrrred完成签到,获得积分10
12秒前
南宫连虎发布了新的文献求助10
14秒前
传奇3应助cc采纳,获得10
17秒前
37秒前
量子星尘发布了新的文献求助10
37秒前
longh发布了新的文献求助20
39秒前
cc发布了新的文献求助10
41秒前
aa发布了新的文献求助10
42秒前
lucky完成签到 ,获得积分10
43秒前
123完成签到,获得积分10
52秒前
53秒前
53秒前
fat完成签到,获得积分10
56秒前
123发布了新的文献求助10
57秒前
脑洞疼应助lf采纳,获得10
1分钟前
健壮的花瓣完成签到 ,获得积分10
1分钟前
oywt发布了新的文献求助10
1分钟前
霸气鞯完成签到 ,获得积分10
1分钟前
1分钟前
lf发布了新的文献求助10
1分钟前
1分钟前
1分钟前
1分钟前
bbdd2334发布了新的文献求助10
1分钟前
1分钟前
1分钟前
李健的小迷弟应助bbdd2334采纳,获得10
1分钟前
1分钟前
忧伤的风华完成签到,获得积分10
1分钟前
thanhvader999完成签到,获得积分10
1分钟前
小乘号子发布了新的文献求助10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
1分钟前
小马甲应助科研通管家采纳,获得10
1分钟前
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976600
求助须知:如何正确求助?哪些是违规求助? 3520700
关于积分的说明 11204482
捐赠科研通 3257320
什么是DOI,文献DOI怎么找? 1798683
邀请新用户注册赠送积分活动 877881
科研通“疑难数据库(出版商)”最低求助积分说明 806613