Quantitative Comparison and Chemical Profile Analysis of Different Medicinal Parts of Perilla frutescens (L.) Britt. from Different Varieties and Harvest Periods

紫苏 化学 气相色谱法 气相色谱-质谱法 药用植物 质谱法 化学成分 色谱法 园艺 植物 食品科学 生物
作者
Yuqi Fan,Xiunan Cao,Mengmeng Zhang,Shujie Wei,Yameng Zhu,Huizi Ouyang,Jun He
出处
期刊:Journal of Agricultural and Food Chemistry [American Chemical Society]
卷期号:70 (28): 8838-8853 被引量:24
标识
DOI:10.1021/acs.jafc.2c03104
摘要

Perilla frutescens (L.) Britt. is a plant that has been classified as one of the "One Root of Medicine and Food", and it can be used both as medicine and as food. To explore the influence of different varieties and harvest periods on the quality of different medicinal parts of P. frutescens, a comprehensive study on the chemical constituents of P. frutescens based on plant metabolomics was conducted. A total of 57 nonvolatile chemical components and 105 volatile chemical components of P. frutescens were characterized by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) and gas chromatography-mass spectrometry (GC-MS). Furthermore, 35, 27, and 2 nonvolatile constituents as well as 16, 16, and 18 volatile constituents were identified as potential markers for discriminating P. frutescens between different medicinal parts, different varieties, and different harvest periods, respectively. Besides, 22 bioactive compounds of P. frutescens were quantitatively determined by a new sensitive UPLC-MS/MS method. This study comprehensively compares the differences and similarities of P. frutescens among the different medicinal parts, different varieties, and different harvest periods, and the results of this study may provide a theoretical basis and guidance for studying the quality evaluation and the optimization of the harvest period of this plant.
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