组织蛋白酶B
生物医学中的光声成像
分子成像
微气泡
细胞内
组织蛋白酶
癌症研究
体内
化学
医学
超声波
生物化学
放射科
生物
酶
光学
物理
生物技术
作者
Chenchen Wang,Wei Du,Chenfan Wu,Shan Dan,Miao Sun,Tong Zhang,Bin Wang,Yue Yuan,Gaolin Liang
标识
DOI:10.1002/anie.202114766
摘要
Abstract Cathepsin B (CTSB) is a lysosomal protease that is overexpressed in the early stage of many cancer types. Precise evaluation of CTSB expression in vivo may provide a promising method for the early diagnosis of cancers. By virtue of the high‐resolution PA imaging modality, a “smart” photoacoustic (PA) probe Cypate‐CBT , which can self‐assemble to cypate‐containing nanoparticles in response to abundant GSH and CTSB inside tumor cells, was developed for the sensitive and specific detection of CTSB activity. Compared with unmodified Cypate, our probe Cypate‐CBT exhibited a 4.9‐fold or 4.7‐fold PA signal enhancement in CTSB‐overexpressing MDA‐MB‐231 cancer cells or tumors, respectively, revealing intracellular accumulation of the probe after CTSB‐initiated self‐assembly. We expect Cypate‐CBT to be employed as an effective PA imaging agent for clinical diagnosis of cancer at early stages.
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