BCL6公司
生发中心
CXCL13型
淋巴瘤
表型
血管免疫母细胞性T细胞淋巴瘤
免疫分型
滤泡性淋巴瘤
生物
病理
免疫学
癌症研究
抗体
B细胞
医学
流式细胞术
T细胞
趋化因子
遗传学
基因
炎症
免疫系统
趋化因子受体
作者
Matthew Koo,Jingjing Zhang,Brent Tan,Jason H. Kurzer,Dita Gratzinger,Shuchun Zhao,Carlos J. Suarez,Izidore S. Lossos,Roger A. Warnke,Yasodha Natkunam
标识
DOI:10.1097/pas.0000000000001852
摘要
The diagnosis of angioimmunoblastic T-cell lymphoma (AITL) is complex and requires the demonstration of a T-follicular helper (TFH) phenotype. Immunophenotypic markers that detect the TFH phenotype are highly variable, thereby necessitating the use of 3 to 5 TFH markers to substantiate a TFH phenotype. We tested the utility of germinal center markers human germinal center–associated lymphoma (HGAL) and LIM-domain only 2 (LMO2) in detecting a TFH phenotype. We compared their staining to that of 6 TFH markers in current use, PD-1, ICOS, CXCL13, SAP, CD10, and BCL6, in a cohort of 23 AITL. Our results show that although both markers can detect a TFH phenotype, HGAL was superior to LMO2 in the percent of cells stained and the intensity of staining, 2 variables used to generate H -scores. Using H -scores as the metric, HGAL was most comparable to BCL6 among the currently used TFH markers and was more sensitive than CXCL13, SAP, CD10, and LMO2. PD-1 and ICOS emerged as the most robust of the 8 markers tested in this study in detecting a TFH phenotype. We conclude that HGAL is a reliable marker of TFH cells and can aid in the diagnosis of lymphomas of TFH derivation, particularly in the recognition of early patterns of AITL.
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