双金属片
基质(水族馆)
过氧化氢
纳米颗粒
纳米技术
石墨烯
化学
电化学
催化作用
电极
材料科学
化学工程
有机化学
地质学
工程类
物理化学
海洋学
作者
Euna Ko,Van‐Khue Tran,Gi Hun Seong,Won Hur,Hyun Seok Choi,Gi Hun Seong
标识
DOI:10.1016/j.snb.2019.05.051
摘要
Nanostructured artificial enzymes, known as nanozymes, have been considered effective alternatives for natural enzymes with the attractive advantages. Herein, bimetallic Au and Pt nanoparticles with graphene oxide (GO) were immobilized chemically on the surface of agarose microbeads. Due to the synergistic effect of the bimetallic nanoparticles and GO ([email protected]/GO), this hybrid nanostructure exhibited strong peroxidase-like catalytic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) substrate in the presence of H2O2. Colorimetric determination of H2O2, indicated by the blue color of the oxidized TMB within 1 min, was observed using the [email protected]/GO nanozymes. To demonstrate practical application of the nanozymes, the [email protected]/GO microbeads were packed into a film-based electrochemical point-of-care (POC) device for sensitive and rapid H2O2 detection. Upon introduction of TMB substrate solution containing H2O2, the catalytically oxidized TMB was electrochemically reduced on the electrode surfaces, resulting in a broader detection range of H2O2 (1 μM – 3 mM) and a lower LOD (1.62 μM) than achievable with the colorimetric detection method. Moreover, the developed POC devices showed the capability of accurate determination of H2O2 with strong repeatability and reproducibility in real sample test using artificial urine.
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