Copper‐ Based Metal‐Organic Frameworks as Peroxidase Mimics Leading to Sensitive H2O2 and Glucose Detection

Abstract A copper based metal organic framework (MOF) synthesized under solvo‐thermal conditions was found to exhibit intrinsic peroxidase‐like activity and was established both spectrophotometrically and electrochemically. It was found that the catalytic behaviour had been consistent with Michaelis‐Menten kinetics with a Km value of 0.08 mM with H 2 O 2 , relatively lower than that of Horseradish peroxidase enzyme (HRP) ie; 3.7 mM. The suitability of Cu–MOF for sensing H 2 O 2 was investigated by electrochemical means. In the case of electrochemical studies, the Cu–MOF modified GCE (glassy carbon electrode) showed a very high sensitivity of 263 μA mmol −1 cm −2 with a detection limit up to 35 mM for H 2 O 2 . The peroxidase mimics can be combined with the enzymatic oxidation of glucose for sensing glucose. The detection of H 2 O 2 and glucose using Cu–MOF was found to be highly selective towards the common interfering species.