生物
底漆(化妆品)
克隆(编程)
集合(抽象数据类型)
协议(科学)
遗传学
计算生物学
计算机科学
物理
程序设计语言
医学
热力学
病理
替代医学
作者
Jiantao Tan,Qi Gong,Suize Yu,Yuke Hou,Dongchang Zeng,Qinlong Zhu,Yao‐Guang Liu
标识
DOI:10.1016/j.jgg.2019.05.002
摘要
Here we describe a protocol for wristwatch PCR, an approach based on wristwatch-like structure formed between walking primers to obtain unknown flanks. We specify the criteria for designing wristwatch primers and gene-specific primers. We detail how to set wristwatch primer permutations to obtain personalized walking outcomes and improve walking efficiency. We describe experimental procedures for isolating a DNA of interest using three rounds of nested wristwatch PCR as well as the subsequent steps for DNA purification, cloning, and sequencing.For complete details on the use and execution of this protocol, please refer to Wang et al. (2022).1
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