Methodological Research on Measuring Chlorella Quantity by Spectrophotometry

[Objective] The research aimed to ascertain the wavelength suitable for determining the concn.of chlorella suspension by spectrophotometry.[Method] The absorbance of the suspension with different concn.of Chlorella vulgaris were determined at different wavelengths and the biological statistic analysis was performed on the relevant data.[Result] In the concn.range of 8.7×106-8.7×108/ml,the maximum adsorption wavelength of chlorella suspension was about 680 nm and the absorbance of chlorella suspension with higher concn.exceeded the determination range.The significance test showed that the concn.of chlorella suspension showed extremely significant linear correlation with its absorbance at 9 wavelengths.The determination sensitivity at the wavelength of 680 nm was highest and its highest suspension concn.was lowest,being 3.79×108/ml.The determination sensitivity at the wavelength of 700 nm was lowest and its highest suspension concn.was highest,being 7.7×108/ml.The determination sensitivity at the wavelength of 560 nm was just higher than that at the wavelength of 700 nm and its highest suspension concn.was 6.9×108/ml.The determination sensitivity at the wavelength of 540 nm was higher than that at the wavelength of 560 nm and its highest suspension concn.was 6.5×108/ml.[Conclusion] The determination on the concn.of chlorella suspension at the wavelength of 680 nm had high sensitivity and more precise data.