旁分泌信号
干细胞
血管生成
细胞疗法
分泌物
干细胞疗法
医学
细胞生物学
趋化因子
癌症研究
生物
免疫学
内科学
炎症
受体
作者
Bernhard Wernly,Inês C. Gonçalves,Attila Kiss,Vera Paar,Tobias Mösenlechner,Michael Leisch,D. Santer,Lucas Motloch,Klaus Ulrich Klein,Eva Verena Tretter,Daniel Kretzschmar,Bruno K. Podesser,Christian Jung,Uta C. Hoppe,Michael Lichtenauer
标识
DOI:10.1002/biot.201600732
摘要
Stem cell therapy for acute myocardial infarction (AMI) seemed to be a promising therapy, however, large clinical trials brought differential outcome. It has been shown that paracrine effects of secretomes of stem cells rather than cell therapy might play a fundamental role. The present study seeks to compare cell processing protocols of clinical trials and investigate effects of differential cell culture conditions on chemokine secretion and functional effects. Different secretomes are compared regarding IL-8, VEGF, MCP-1, and TNF-alpha secretion. Secretome mediated effects are evaluated on endothelial cell (HUVEC) tube formation and migration. Cardioprotective signaling kinases in human cardiomyocytes are determined by Western immunoblotting. Cells processed according to the REPAIR-AMI protocol secrete significantly higher amounts of IL-8 (487.3 ± 1231.1 vs 9.1 ± 8.2 pg mL-1 ; p < 0.05). REAPIR-AMI supernatants lead to significantly pronounced tube formation and migration on HUVEC and enhance the phosphorylation of Akt, ERK, and CREB. Cell processing conditions have a major impact on the composition of the secretome. The REPAIR-AMI secretome significantly enhances proangiogenic chemokine secretion, angiogenesis, cell migration, and cardioprotective signaling pathways. These results might explain differential outcomes between clinical trials. Optimizing cell processing protocols with special regards to paracrine factors, might open a new therapeutic concept for improving patient outcome.
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