会合(天文学)
生物
计算生物学
基因
聚合酶链反应
实时聚合酶链反应
计算机科学
化学
生物化学
物理
天文
作者
Fatjon Leti,Johanna K. DiStefano
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 257-265
被引量:10
标识
DOI:10.1007/978-1-4939-7471-9_14
摘要
MicroRNAs are small noncoding RNAs that function to regulate gene expression. In general, miRNAs are posttranscriptional regulators that imperfectly bind to the 3'untranslated region (3'UTR) of target mRNAs bearing complementary sequences, and target more than half of all protein-coding genes in the human genome. The dysregulation of miRNA expression and activity has been linked with numerous diseases, including cancer, cardiovascular diseases, neurodegenerative disorders, and diabetes. To better understand the relationship between miRNAs and human disease, a variety of techniques have been used to measure and validate miRNA expression in many cells, tissues, body fluids, and organs. For many years, quantitative polymerase chain reaction (qPCR) has been the gold standard for measuring relative gene expression, and is now also widely used to assess miRNA abundance. In this chapter, we describe a quick protocol for miRNA extraction, reverse transcription, qPCR, and data analysis.
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