RNA N6‐methyladenosine methyltransferase‐like 3 promotes liver cancer progression through YTHDF2‐dependent posttranscriptional silencing of SOCS2

生物 基因敲除 癌症研究 基因沉默 表观遗传学 核糖核酸 甲基转移酶 染色质重塑 细胞生物学 分子生物学 甲基化 细胞培养 基因 遗传学
作者
Mengnuo Chen,Lai Wei,Cheuk‐Ting Law,Felice Ho‐Ching Tsang,Jialing Shen,Carol Lai‐Hung Cheng,Long‐Hin Tsang,Daniel Wai‐Hung Ho,David Kung‐Chun Chiu,Joyce Man‐Fong Lee,Carmen Chak‐Lui Wong,Irene Oi‐Lin Ng,Chun–Ming Wong
出处
期刊:Hepatology [Wiley]
卷期号:67 (6): 2254-2270 被引量:1010
标识
DOI:10.1002/hep.29683
摘要

Epigenetic alterations have contributed greatly to human carcinogenesis. Conventional epigenetic studies have predominantly focused on DNA methylation, histone modifications, and chromatin remodeling. Recently, diverse and reversible chemical modifications of RNAs have emerged as a new layer of epigenetic regulation. N6‐methyladenosine (m6A) is the most abundant chemical modification of eukaryotic messenger RNA (mRNA) and is important for the regulation of mRNA stability, splicing, and translation. Using transcriptome sequencing, we discovered that methyltransferase‐like 3 (METTL3), a major RNA N6‐adenosine methyltransferase, was significantly up‐regulated in human hepatocellular carcinoma (HCC) and multiple solid tumors. Clinically, overexpression of METTL3 is associated with poor prognosis of patients with HCC. Functionally, we proved that knockdown of METTL3 drastically reduced HCC cell proliferation, migration, and colony formation in vitro . Knockout of METTL3 remarkably suppressed HCC tumorigenicity and lung metastasis in vivo . On the other hand, using the CRISPR/dCas9‐VP64 activation system, we demonstrated that overexpression of METTL3 significantly promoted HCC growth both in vitro and in vivo . Through transcriptome sequencing, m6A sequencing, and m6A methylated RNA immuno‐precipitation quantitative reverse‐transcription polymerase chain reaction, we identified suppressor of cytokine signaling 2 (SOCS2) as a target of METTL3‐mediated m6A modification. Knockdown of METTL3 substantially abolished SOCS2 mRNA m6A modification and augmented SOCS2 mRNA expression. We also showed that m6A‐mediated SOCS2 mRNA degradation relied on the m6A reader protein YTHDF2‐dependent pathway. Conclusion : METTL3 is frequently up‐regulated in human HCC and contributes to HCC progression. METTL3 represses SOCS2 expression in HCC through an m6A‐YTHDF2‐dependent mechanism. Our findings suggest an important mechanism of epigenetic alteration in liver carcinogenesis. (H epatology 2018;67:2254‐2270).
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