The corrugated web composite box-girder (CWCB) is a novel structural form and has been widely adopted in bridge engineering due to its excellent mechanical properties and construction convenience. However, researches on the temperature field in CWCB is very limited, which may augment the risk of concrete cracking and steel web bucking and deteriorate the durability of bridge structures. This paper thereby experimentally investigates the time-varying and spatial temperature distribution features of CWCB based on a long-term temperature monitoring system. The experimental results indicate that the temperature field in CWCB is non-uniformly distributed and the vertical and horizontal temperature differences can reach up to 13.4 ℃ and 19.1 ℃, respectively. Meanwhile, the observed temperature distribution profile of the experimental CWCB is varying with seasons owing to the different exposure to solar radiation. Then, a refined numerical thermal simulation approach is developed and its reliability and accuracy have been validated by the experimental temperature data of three types of box-girders, i.e., CWCB, ordinary composite box-girder (OCB), and conventional concrete box-girder (CCB). Based on the validated numerical approach, the temperature fields of three full-sized box-girders are therewith compared and the results demonstrate that the temperature field in CWCB is much more complicated than the traditional box-girders. Besides, the obtained cross-sectional temperature differences of CWCB are significantly larger than that of the traditional box-girders. Finally, two temperature profiles have been proposed to characterize the thermal actions in CWCB, which are composed of parabolas and polylines. The outcome of this study can provide a useful tool for analyzing the temperature field and calculating the design thermal load of CWCB.