Design of Fmoc-Phenylalanine Nanofibrillar Hydrogel and Mechanistic Studies of Its Antimicrobial Action against Both Gram-Positive and Gram-Negative Bacteria

In pursuit of efficient antimicrobial agents, biomaterials such as hydrogels have drawn a considerable amount of attention due to their numerous advantages such as a high degree of hydration, biocompatibility, stability, and direct application at an infectious site. Particularly, biomaterials such as hydrogels based on Fmoc-protected peptides and amino acids have proven to be immensely advantageous. Such biomaterials can undergo gelation by simple pH modulation and can be used for various biological applications. Keeping this in mind, in this work, we reported the synthesis of Fmoc-phenylalanine (Fmoc-F)-based hydrogels using trisodium citrate as a pH modulator and compared them with the previously reported pH modulator glucono-δ-lactone. The gels were compared using various characterization techniques such as rheometry, field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), small angle X-ray scattering (SAXS), FT-IR, thioflavin T (ThT) binding assay, and zeta potential studies. These studies highlighted the role of pH modulators in affecting various parameters such as the ability to alter the zeta potential of the nanofibrils, improve their bactericidal action, reduce the amyloidic characters, shift the lattice packing from amorphous to crystalline, and introduce fluorescence and thermoreversibility. Interestingly, this is the first report where the Fmoc-F-based hydrogel has been shown to be effective against Gram-negative bacteria along with Gram-positive bacteria as well. Additionally, the mechanism of antimicrobial action was investigated using docking and antioxidant studies.