Predictive potentials of glycosylation-related genes in glioma prognosis and their correlation with immune infiltration

胶质瘤 免疫系统 生物 糖基化 基因 癌症研究 计算生物学 免疫学 遗传学
作者
Yifei Sun,Lanchun Zhang,Rui‐Ze Niu,Li Chen,Qingjie Xia,Liu‐Lin Xiong,Ting‐Hua Wang
出处
期刊:Scientific Reports [Springer Nature]
卷期号:14 (1) 被引量:2
标识
DOI:10.1038/s41598-024-51973-0
摘要

Abstract Glycosylation is currently considered to be an important hallmark of cancer. However, the characterization of glycosylation-related gene sets has not been comprehensively analyzed in glioma, and the relationship between glycosylation-related genes and glioma prognosis has not been elucidated. Here, we firstly found that the glycosylation-related differentially expressed genes in glioma patients were engaged in biological functions related to glioma progression revealed by enrichment analysis. Then seven glycosylation genes ( BGN , C1GALT1C1L , GALNT13 , SDC1 , SERPINA1 , SPTBN5 and TUBA1C ) associated with glioma prognosis were screened out by consensus clustering, principal component analysis, Lasso regression, and univariate and multivariate Cox regression analysis using the TCGA-GTEx database. A glycosylation-related prognostic signature was developed and validated using CGGA database data with significantly accurate prediction on glioma prognosis, which showed better capacity to predict the prognosis of glioma patients than clinicopathological factors do. GSEA enrichment analysis based on the risk score further revealed that patients in the high-risk group were involved in immune-related pathways such as cytokine signaling, inflammatory responses, and immune regulation, as well as glycan synthesis and metabolic function. Immuno-correlation analysis revealed that a variety of immune cell infiltrations, such as Macrophage, activated dendritic cell, Regulatory T cell (Treg), and Natural killer cell, were increased in the high-risk group. Moreover, functional experiments were performed to evaluate the roles of risk genes in the cell viability and cell number of glioma U87 and U251 cells, which demonstrated that silencing BGN , SDC1 , SERPINA1 , TUBA1C , C1GALT1C1L and SPTBN5 could inhibit the growth and viability of glioma cells. These findings strengthened the prognostic potentials of our predictive signature in glioma. In conclusion, this prognostic model composed of 7 glycosylation-related genes distinguishes well the high-risk glioma patients, which might potentially serve as caner biomarkers for disease diagnosis and treatment.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
吃鸡蛋不吃鸡蛋黄完成签到,获得积分10
刚刚
iamchens发布了新的文献求助10
刚刚
1秒前
1秒前
llg发布了新的文献求助10
2秒前
3秒前
4秒前
NexusExplorer应助畅彤采纳,获得10
4秒前
Ava应助秋光采纳,获得10
6秒前
乐观小蕊发布了新的文献求助10
7秒前
胖飞飞完成签到,获得积分10
7秒前
7秒前
潘科学家发布了新的文献求助10
8秒前
gery完成签到,获得积分10
10秒前
10秒前
Singularity应助zzz采纳,获得10
10秒前
爆米花应助子然采纳,获得10
10秒前
11秒前
zhiqing发布了新的文献求助10
12秒前
12秒前
13秒前
港a发布了新的文献求助10
13秒前
豆子应助lijingwen采纳,获得10
13秒前
13秒前
NexusExplorer应助lijingwen采纳,获得10
13秒前
爆米花应助lijingwen采纳,获得10
13秒前
rosalieshi应助lijingwen采纳,获得30
13秒前
cq220完成签到,获得积分10
14秒前
15秒前
15秒前
Owen应助liclic采纳,获得10
15秒前
15秒前
15秒前
所所应助llg采纳,获得10
15秒前
无聊的蛋挞完成签到,获得积分10
16秒前
16秒前
16秒前
16秒前
16秒前
乐乐应助研猫采纳,获得10
17秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
Sustainability in ’Tides Chemistry 1500
Studien zur Ideengeschichte der Gesetzgebung 1000
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3071903
求助须知:如何正确求助?哪些是违规求助? 2725788
关于积分的说明 7491264
捐赠科研通 2373147
什么是DOI,文献DOI怎么找? 1258476
科研通“疑难数据库(出版商)”最低求助积分说明 610277
版权声明 596944