Tong-Xie-Yao-Fang promotes dendritic cells maturation and retards tumor growth in colorectal cancer mice with chronic restraint stress

医学 结直肠癌 CD8型 慢性应激 CD80 免疫学 癌症 尾部悬挂试验 内科学 免疫系统 行为绝望测验 细胞毒性T细胞 生物 CD40 体外 抗抑郁药 生物化学 海马体
作者
Yifang Jiang,Yane Hu,Yi Yang,Ran Yan,Lili Zheng,Xi Fu,Chong Xiao,Fengming You
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:319: 117069-117069 被引量:15
标识
DOI:10.1016/j.jep.2023.117069
摘要

Depression is one of the important risk factors that accelerate the progression of colorectal cancer (CRC). Tong-Xie-Yao-Fang (TXYF) is a widely used classical formula for treating psychiatric-related intestinal diseases in traditional Chinese medicine, that is composed of four different herbs: Atractylodes macrocephala Koidz. (Baizhu), Paeonia lactiflora Pall. (Baishaoyao), Citrus reticulata Blanco (Chenpi), Saposhnikovia divaricata (Turcz.) Schischk (Fangfeng). TXYF has over a hundred years of history and can significantly improve depression and reduce intestinal symptoms. However, the intervention effect and mechanism of TXYF on colorectal cancer accompanied by psychological stress are not still clear.This study investigated the therapeutic effect of TXYF on CRC mice with chronic restraint stress (CRS) and to explore its mechanism.We constructed a mouse model of chronic stress by CRS and subcutaneous injection of CT26-Luc cells, and administered TXYF by gavage. We measured the body weight, tumor size, and tumor weight of each group of mice. The tumor growth was monitored dynamically of by vivo bioluminescence analysis. The depressive state of each group of mice were evaluated by tail suspension test, forced swimming test, and hormone level changes. We used flow cytometry to detect the ratio of CD4+ T cells, CD8+ T cells, Th1 cells, Th2 cells, and dendritic cells (DCs) phenotype (MHC II, CD80, and CD86) and chemotaxis ability (CXCR4 and CCR7) of in peripheral blood and tumor tissue. the levels of IL-12, IL-18, Th1 cytokines, and Th2 cytokines in the serum of each group of mice were determined by ELISA.TXYF can improve the body weight of CRC mice with CRS, inhibit tumor volume and weight, alleviate depressive state, upregulate 5-HT levels, and inhibit HPA axis hormone secretion. The results of flow cytometry showed that TXYF can promote the maturation of DCs phenotype and function, enhance antigen presentation ability, increase the ratio of CD4+ T cells and CD4+/CD8+ T cells, and shift Th1/Th2 balance towards Th1 cells, thus increasing serum levels of IFN-γ, IL-18, IL-2, and IL-12, while decreasing serum levels of IL-4 and IL-10, and effectively triggering T cell-mediated immune response.This study shows that TXYF inhibits the growth of tumors in CRC mice with CRS by stimulating immune response. The mechanism may be inhibiting the HPA axis and promoting DCs maturation, thus activating T cells and enhancing anti-tumor immune response, ultimately preventing the progression of CRC.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
天天呼的海角完成签到,获得积分10
2秒前
量子星尘发布了新的文献求助10
5秒前
5秒前
5秒前
jiangcai完成签到,获得积分10
6秒前
Cherry完成签到,获得积分10
8秒前
正能量的可可可完成签到,获得积分10
8秒前
tuo zhang发布了新的文献求助10
11秒前
草莓养乐多完成签到 ,获得积分10
11秒前
糊涂完成签到,获得积分10
11秒前
哦哦哦发布了新的文献求助10
11秒前
in完成签到 ,获得积分10
12秒前
pilot完成签到,获得积分10
14秒前
劣根完成签到,获得积分10
15秒前
Liang发布了新的文献求助20
15秒前
17秒前
18秒前
曾小莹完成签到,获得积分10
18秒前
苏信怜完成签到,获得积分10
19秒前
22秒前
大气灵枫发布了新的文献求助10
22秒前
nn发布了新的文献求助10
23秒前
tuo zhang完成签到,获得积分10
25秒前
务实的绝悟完成签到,获得积分10
25秒前
怡然问晴发布了新的文献求助10
27秒前
开拖拉机的医学僧完成签到 ,获得积分10
28秒前
体贴凌柏发布了新的文献求助10
29秒前
Kinn完成签到,获得积分10
30秒前
30秒前
华仔应助枕星采纳,获得10
30秒前
Perry应助科研通管家采纳,获得30
31秒前
Jasper应助科研通管家采纳,获得10
31秒前
Jasper应助科研通管家采纳,获得30
31秒前
共享精神应助科研通管家采纳,获得30
31秒前
32秒前
无花果应助科研通管家采纳,获得10
32秒前
小二郎应助科研通管家采纳,获得10
32秒前
CipherSage应助科研通管家采纳,获得10
32秒前
bkagyin应助科研通管家采纳,获得10
32秒前
32秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038303
求助须知:如何正确求助?哪些是违规求助? 3576013
关于积分的说明 11374210
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029