亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Tong-Xie-Yao-Fang promotes dendritic cells maturation and retards tumor growth in colorectal cancer mice with chronic restraint stress

医学 结直肠癌 CD8型 慢性应激 CD80 免疫学 癌症 尾部悬挂试验 内科学 免疫系统 行为绝望测验 细胞毒性T细胞 生物 CD40 体外 抗抑郁药 生物化学 海马体
作者
Yifang Jiang,Yane Hu,Yi Yang,Ran Yan,Lili Zheng,Xi Fu,Chong Xiao,Fengming You
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:319: 117069-117069 被引量:9
标识
DOI:10.1016/j.jep.2023.117069
摘要

Depression is one of the important risk factors that accelerate the progression of colorectal cancer (CRC). Tong-Xie-Yao-Fang (TXYF) is a widely used classical formula for treating psychiatric-related intestinal diseases in traditional Chinese medicine, that is composed of four different herbs: Atractylodes macrocephala Koidz. (Baizhu), Paeonia lactiflora Pall. (Baishaoyao), Citrus reticulata Blanco (Chenpi), Saposhnikovia divaricata (Turcz.) Schischk (Fangfeng). TXYF has over a hundred years of history and can significantly improve depression and reduce intestinal symptoms. However, the intervention effect and mechanism of TXYF on colorectal cancer accompanied by psychological stress are not still clear.This study investigated the therapeutic effect of TXYF on CRC mice with chronic restraint stress (CRS) and to explore its mechanism.We constructed a mouse model of chronic stress by CRS and subcutaneous injection of CT26-Luc cells, and administered TXYF by gavage. We measured the body weight, tumor size, and tumor weight of each group of mice. The tumor growth was monitored dynamically of by vivo bioluminescence analysis. The depressive state of each group of mice were evaluated by tail suspension test, forced swimming test, and hormone level changes. We used flow cytometry to detect the ratio of CD4+ T cells, CD8+ T cells, Th1 cells, Th2 cells, and dendritic cells (DCs) phenotype (MHC II, CD80, and CD86) and chemotaxis ability (CXCR4 and CCR7) of in peripheral blood and tumor tissue. the levels of IL-12, IL-18, Th1 cytokines, and Th2 cytokines in the serum of each group of mice were determined by ELISA.TXYF can improve the body weight of CRC mice with CRS, inhibit tumor volume and weight, alleviate depressive state, upregulate 5-HT levels, and inhibit HPA axis hormone secretion. The results of flow cytometry showed that TXYF can promote the maturation of DCs phenotype and function, enhance antigen presentation ability, increase the ratio of CD4+ T cells and CD4+/CD8+ T cells, and shift Th1/Th2 balance towards Th1 cells, thus increasing serum levels of IFN-γ, IL-18, IL-2, and IL-12, while decreasing serum levels of IL-4 and IL-10, and effectively triggering T cell-mediated immune response.This study shows that TXYF inhibits the growth of tumors in CRC mice with CRS by stimulating immune response. The mechanism may be inhibiting the HPA axis and promoting DCs maturation, thus activating T cells and enhancing anti-tumor immune response, ultimately preventing the progression of CRC.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Boren发布了新的文献求助10
2秒前
大个应助Boren采纳,获得10
14秒前
Raunio完成签到,获得积分10
16秒前
Akitten发布了新的文献求助10
29秒前
完美世界应助lhy采纳,获得10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
Boren发布了新的文献求助10
1分钟前
lhy发布了新的文献求助10
1分钟前
董可以发布了新的文献求助10
1分钟前
风吹而过完成签到 ,获得积分10
1分钟前
乐乐应助董可以采纳,获得10
1分钟前
2分钟前
2分钟前
liufan完成签到 ,获得积分10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
打打应助科研通管家采纳,获得30
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
3分钟前
3分钟前
杪夏二八完成签到 ,获得积分10
4分钟前
souther完成签到,获得积分0
4分钟前
乐乐应助Akitten采纳,获得10
4分钟前
4分钟前
kangkang发布了新的文献求助10
4分钟前
传奇完成签到 ,获得积分10
5分钟前
jyy关闭了jyy文献求助
5分钟前
丘比特应助怕孤单的思雁采纳,获得10
5分钟前
5分钟前
5分钟前
Akitten发布了新的文献求助10
5分钟前
5分钟前
Li完成签到,获得积分10
6分钟前
6分钟前
自然的衫完成签到 ,获得积分10
6分钟前
6分钟前
阿巴阿巴茶完成签到,获得积分10
7分钟前
lhy发布了新的文献求助10
7分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990332
求助须知:如何正确求助?哪些是违规求助? 3532146
关于积分的说明 11256483
捐赠科研通 3271042
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234