汤剂
熊果酸
化学
甜叶菊
传统医学
餐后
糖苷
IC50型
甜菊
食品科学
色谱法
生物化学
立体化学
生物
胰岛素
医学
体外
内分泌学
替代医学
病理
作者
Sofía Padilla‐Mayne,Berenice Ovalle‐Magallanes,Mario Figueroa,Edelmira Linares,Robert Bye,Isabel Rivero‐Cruz,Martín González‐Andrade,Rodrigo Aguayo‐Ortiz,Rachel Mata
标识
DOI:10.1021/acs.jnatprod.3c00711
摘要
A decoction of the roots (31.6–316 mg/kg) from Stevia serrata Cav. (Asteraceae) as well as the main component (5–150 mg/kg) showed hypoglycemic and antihyperglycemic effects in mice. The fractionation of the active extract led to the isolation of dammaradiene acetate (1), stevisalioside A (2), and three new chemical entities characterized by spectroscopic methods and named stevisaliosides B–D (3–5). Glycoside 2 (5 and 50 mg/kg) decreased blood glucose levels and the postprandial peak during oral glucose and insulin tolerance tests in STZ-hyperglycemic mice. Compounds 1–5 were tested also against PTP1B1–400 and showed IC50 values of 1180.9 ± 0.33, 526.8 ± 0.02, 532.1 ± 0.03, 928.2 ± 0.39, and 31.8 ± 1.09 μM, respectively. Compound 5 showed an IC50 value comparable to that of ursolic acid (IC50 = 30.7 ± 0.00 μM). Docking studies revealed that 2–5 and their aglycones bind to PTP1B1–400 in a pocket formed by the C-terminal region. The volatilome of S. serrata was characterized by a high content of (E)-longipinene, spathulenol, guaiadiene, seychellene, and aromandendrene. Finally, a UHPLC-UV method was developed and validated to quantify the content of 2 in the decoction of the plant.
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