Chemoenzymatic cascade synthesis of metal‐chelating α‐amino acids

Metal chelating non‐canonical amino acids (ncAAs) are uniquely functional building blocks for proteins, peptide catalysts, and small molecule sensors. However, catalytic asymmetric approaches to synthesizing these molecules is hindered by their functional group variability and intrinsic propensity to ligate metals. In particular, bipyridyl‐L‐alanine (BpyAla) is a highly sought ncAA but its complex, inefficient syntheses has limited utility. Here, we develop a chemoenzymatic approach to efficiently construct BpyAla. Three enzymes that can be produced in high titer together react to convert Gly and an aldehyde into the corresponding β‐hydroxy ncAA, which is subsequently deoxygenated. We explore approaches to synthesizing bi‐aryl aldehydes and show how the three‐enzymatic cascade can access a range of α‐amino acids with bulky side chains, including a variety of metal chelating amino acids. We show that newly‐accessible BpyAla analogs are compatible with existing amber suppression technology, which will enable future merging of traditional synthetic and biosynthetic approaches to tuning metal reactivity.