丙炔基转移酶
二酮哌嗪
化学
预酸化
异源表达
吲哚试验
生物化学
计算生物学
基因
酶
立体化学
生物
重组DNA
作者
Xiaotian Yu,Chuanteng Ma,Wenxue Wang,Jing Ge,Zian Wang,Jiaqi Lin,Qian Che,Guojian Zhang,Tianjiao Zhu,Dehai Li
出处
期刊:Organic Letters
[American Chemical Society]
日期:2024-04-12
卷期号:26 (16): 3349-3354
标识
DOI:10.1021/acs.orglett.4c00714
摘要
UbiA-type prenyltransferases (PTases) are significant enzymes that lead to structurally diverse meroterpenoids. Herein, we report the identification and characterization of an undescribed UbiA-type PTase, FtaB, that is responsible for the farnesylation of indole-containing diketopiperazines (DKPs) through genome mining. Heterologous expression of the fta gene cluster and non-native pathways result in the production of a series of new C2-farnesylated DKPs. This study broadens the reaction scope of UbiA-type PTases and expands the chemical diversity of meroterpenoids.
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