化学
脱氧核酶
双模
小RNA
对偶(语法数字)
熵(时间箭头)
纳米技术
色谱法
检出限
生物化学
热力学
基因
航空航天工程
艺术
物理
材料科学
文学类
工程类
作者
Jianhong Zhang,Dan Bai,Guoming Xie,Yang Yu,Yu Lin,Yu‐Lei Hou,Ying Yu,Yaoyi Zhang,Rong Zhao,Zhongzhong Wang,Luojia Wang,Hui Chen
出处
期刊:Talanta
[Elsevier]
日期:2024-08-01
卷期号:275: 126123-126123
标识
DOI:10.1016/j.talanta.2024.126123
摘要
Accurate microRNA (miRNA) detection is pivotal in the diagnosis and monitoring of cancer. Entropy-driven catalysis (EDC) has attracted widespread attention as an enzyme-free, isothermal technique for miRNA detection owing to its inherent simplicity and reliability. However, conventional EDC is a single-output mode, limiting the efficiency of signal amplification. In this study, a novel EDC dual-output mode was employed in conjunction with DNAzyme, resulting in the development of an EDC dual-end DNAzyme (EDC-DED) approach for highly sensitive miRNA detection. In this system, miRNA-21 initiated the EDC reaction, producing a large amount of catalytically active dual-end Mg2+-dependent DNAzyme. The DNAzyme further cleaved the reporter cyclically, generating a notably amplified fluorescence signal. The proposed method achieved a low detection limit of 2 pM. Compared with the traditional EDC single-end DNAzyme (EDC-SED) strategy, the present method exhibited superior amplification efficiency, enhancing detection sensitivity by approximately 46.5-fold. Furthermore, this platform demonstrated ideal specificity, satisfactory reproducibility and acceptable detection capabilities in clinical serum samples. Therefore, the straightforward and convenient strategy is a potential tool for miRNA analysis, which may provide a new perspective for biological analysis and clinical application.
科研通智能强力驱动
Strongly Powered by AbleSci AI