Phytochemical profile, antioxidant, and wound healing activities of Echium amoenum (Boraginaceae)

Natural products' inflammatory and antioxidant properties have crucial roles in tissue repair and regeneration. This study aimed to investigate HPLC analysis, antioxidant activity, and wound-healing effect of Echium amoenum (EA) on rats. The EA was analysed for the determination of shikonin a derivative of naphthoquinone. In the wound healing assessment, forty Wistar rats were divided into five groups that were receiving the cream base, normal saline (NS), 1% silver sulfadiazine (SSD), and 5 and 10% EA cream (EAC). Shikonin was found in the EA extract. The average wound area on the 10th day was 0.71 ± 0.01, 0.86 ± 0.13, 0.58 ± 0.08, 0.53 ± 0.03 and 0.43 ± 0.04 cm2 for the cream base, NS, SSD, and 5 and 10% EAC, respectively. The collagen fibres were well formed and horizontally oriented in 5 and 10% EAC groups compared to other groups. EA cream was an effective treatment for wound healing in comparison with SSD.